Title | Baculovirus and Insect Cell Expression Protocols [electronic resource] / edited by David W. Murhammer |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2016 |
Edition | 3rd ed. 2016 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-3043-2 |
Descript | XII, 508 p. 65 illus., 15 illus. in color. online resource |
Useful Tips, Widely Used Techniques And Quantifying Cell Metabolic Behavior -- Introduction to Baculovirus Molecular Biology -- Baculovirus Transfer Vectors -- Recombinant Baculovirus Isolation -- Gene Expression In Mammalian Cells Using Bacmam, A Modified Baculovirus System -- Available Lepidopteran Insect Cell Lines -- Lepidopteran Insect Cell Line Isolation from Insect Tissue -- Development Of Serum-Free Media For Lepidopteran Insect Cell Lines -- Routine Maintenance And Storage Of Lepidopteran Insect Cell -- Small-Scale Production Of Recombinant Proteins Using The Baculovirus Expression Vector System -- Recombinant Protein Production In Large Scale Agitated Bioreactors Using The Baculovirus Expression Vector System -- Protein Expression in Insect and Mammalian Cells Using Baculoviruses in Wave Bioreactors -- Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae -- Production Of Virus-Like Particles For Vaccination -- Alternative Strategies for Expressing Multi-Component Protein Complexes in Insect Cells -- Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression -- Stable Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression -- Transforming Lepidopteran Insect Cells for Improved Protein Processing and Expression -- Introduction To The Use Of Baculoviruses As Biological Insecticides -- Baculovirus Insecticide Production In Insect Larvae -- Evaluation Of The Insecticidal Efficacy Of Wild Type And Recombinant Baculoviruses -- Evaluating Baculovirus Infection Using Green Fluorescent Protein and Variants -- Tubular Bioreactor for Probing Baculovirus Infection and Protein Production -- Gene Silencing In Insect Cells Using Rnai -- Using the Baculovirus/Insect Cell System to Study Apoptosis -- Generation of Envelope-Modified Baculoviruses for Gene Delivery into Mammalian Cells