Title | Drosophila Oogenesis [electronic resource] : Methods and Protocols / edited by Diana P. Bratu, Gerard P. McNeil |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2015 |
Edition | 1st ed. 2015 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-2851-4 |
Descript | XI, 245 p. 51 illus., 29 illus. in color. online resource |
Drosophila melanogaster Oogenesis: An Overview -- Basic Techniques in Drosophila Ovary Preparation -- Mosaic Analysis in the Drosophila Melanogaster Ovary -- Genetic Mosaic Analysis of Stem Cell Lineages in the Drosophila Ovary -- Culturing Drosophila Egg Chambers and Investigating Developmental Processes through Live Imaging -- Border Cell Migration: A Model System for Live Imaging and Genetic Analysis of Collective Cell Movement -- Visualizing Microtubule Networks during Drosophila Oogenesis using Fixed and Live ImagingVisualization of Actin Cytoskeletal Dynamics in Fixed and Live Drosophila Egg Chambers -- Single Molecule RNA in situ Hybridization (smFISH) and Immunofluorescence (IF) in the Drosophila Egg Chamber -- Fluorescent In Situ Hybridization of Nuclear Bodies in Drosophila melanogaster Ovaries -- Ultrastructural Analysis of Drosophila Ovaries by Electron Microscopy -- Immuno-Electron Microscopy and Electron Microscopic in situ Hybridization for Visualizing piRNA Biogenesis Bodies in Drosophila Ovaries -- Visualizing Cytoophidia Expression in Drosophila Follicle Cells via Immunohistochemistry.-Detection of Cell Death and Phagocytosis in the Drosophila Ovary -- Analysis of Cell Cycle Switches in Drosophila Oogenesis -- Global Run-On Sequencing (GRO-seq) Library Preparation from Drosophila Ovaries -- Bioinformatics Analysis to Identify RNA-protein Interactions in Oogenesis