|Title||Western Blotting [electronic resource] : Methods and Protocols|
|Author||edited by Biji T. Kurien, R. Hal Scofield|
|Imprint||New York, NY : Springer New York : Imprint: Humana Press, 2015|
|Descript||XIV, 509 p. 140 illus., 65 illus. in color. online resource|
The Early Days of Blotting -- Origins of Protein Blotting -- Western Blotting: Remembrance of Things Past -- Simian Virus 40 and Protein Transfer -- Western Blotting: An Introduction -- From Little Helpers to Automation -- Spectrophotometric Methods to Determine Protein Concentration -- Solubilization of Proteins: The Importance of Lysis Buffer Choice -- Simultaneous Immunoblotting Analysis with Activity Gel Electrophoresis and 2-D Gel Electrophoresis -- Diffusion Blotting: A Rapid and Simple Method for Production of Multiple Blots from a Single Gel -- Multiple Immunoblots by Passive Diffusion of Proteins from a Single SDS-PAGE Gel -- Slice Blotting -- Localizing Proteins by Tissue Printing -- Dot-Immunobinding Assay (Dot-Iba) -- Analysis of Antibody Clonotype by Affinity Immunoblotting -- Glycosaminoglycan Blotting and Detection after Electrophoresis Separation -- A Well-Based Reverse-Phase Protein Array of Formalin-Fixed Paraffin-Embedded Tissue -- Quantitative Computerized Western Blotting in Detail -- Cationic Electrophoresis and Eastern Blotting -- A Miniaturized Blotting System for Simultaneous Detecting of Different Autoantibodies -- Proteomic Expressional Profiling of a Paraffin Embedded Tissue by Multiplex Tissue Immunoblotting -- Post-Staining Electroblotting for Efficient and Reliable Peptide Blotting -- Multistrip Western Blotting: A Tool for Comparative Quantitative Analysis of Multiple Proteins -- Western Blotting using PVDF Membranes and Its Downstream Applications -- Blotting from PhastGel to Membranes by Ultrasound -- Western Blotting of High and Low Molecular Weight Proteins Using Heat -- Membrane Strip Affinity Purification of Autoantibodies -- Strip Immunoblotting of Multiple Antigenic Peptides -- Double-Blotting: A Solution to the Problem of Non-Specific Binding of Secondary Antibodies in Immunoblotting Procedures -- Method for Resolution and Western Blotting of Very Large Proteins Using Agarose Electrophoresis -- Immunodetection of P-Selectin Using an Antibody to its C-Terminal Tag -- Improvements and Variants of the Mutliple Antigen Blot Assay-MABA: An Immunoenzymatic Technique for Simultaneous Antigen and Antibody Screening -- Blotting from Immobilized pH Gradient Gels: Application to Total Cell Lysates -- Immunoprecipitation: Western Blot for Proteins of Low Abundance -- Native Electrophoresis and Western Blot Analysis (NEWeB): Methods and Applications -- Shift-Western Blotting: Separate Analysis of Protein and DNA from Protein-DNA Complexes -- Grid-Immunoblotting -- Detection and Quantification of Protein-Protein Interactions by Far-Western Blotting -- Western Blot Analysis of Adhesive Interactions under Fluid Shear Conditions: The Blot Rolling Assay -- Centrifuge Blotting -- Blotting of Coomassie Blue Stained Proteins from PAGE Gels to Transparencies -- B Cell ELISPOT: For the Identification of Antigen-Specific Antibody Secreting Cells -- T Cell ELISPOT: For the Identification of Specific Cytokine-Secreting T Cells -- Membrane Microplates for One- and Two-Color ELISPOT and FLUOROSPOT Assays -- SDS PAGE to Immunoblot in One Hour -- Single Cell Western Blotting -- Protein Detection by Simple Western™ Analysis -- Western Blotting Using Microfluidics -- Two-Dimensional Gel-Based Protein Standardization Verified by Western Blot Analysis -- Fingerprint Deposition of Nitrocellulose and Polyvinylidene Difluoride Membranes Using Alkaline Phosphatase -- Other Notable Protein Blotting Methods: A Brief Review.
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