Title | Plant Mitochondria [electronic resource] : Methods and Protocols / edited by James Whelan, Monika W. Murcha |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2015 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-2639-8 |
Descript | XXIII, 303 p. 40 illus., 28 illus. in color. online resource |
Isolation of Intact Mitochondria from the Model Plant Species Arabidopsis thaliana and Oryza sativa -- Determining Mitochondrial Transcript Termini for the Study of Transcription Start Sites and Transcript 5’ End Maturation -- Mitochondrial Run-On Transcription Assay Using Biotin Labeling -- In Vitro RNA Uptake Studies in Plant Mitochondria -- In Vitro and In Vivo Protein Uptake Studies in Plant Mitochondria -- Plant Mitochondrial Proteomics -- Identification of Lysine-Acetylated Mitochondrial Proteins and Their Acetylation Sites -- A Flowchart to Analyze Protease Activity in Plant Mitochondria -- Activity Measurements of Mitochondrial Enzymes in Native Gels -- Activity Assay for Plant Mitochondrial Enzymes -- Analysis of Type II NAD(P)H Dehydrogenases -- Assessment of Respiration in Isolated Plant Mitochondria Using Clark Type Electrodes -- Micro-Respiration Measurements in Plants -- Improvements to Define Mitochondrial Metabolomics Using Non Aqueous Fractionation -- Mitochondrial Markers of Programmed Cell Death in Arabidopsis thaliana -- Imaging and Analysis of Mitochondrial Dynamics in Living Cells -- Analysis of Plant Mitochondrial Function Using Fluorescent Protein Sensors -- In Planta Analysis of Leaf Mitochondrial Superoxide and Nitric Oxide -- Databases and Informatics Resources For Analysis of Plant Mitochondria -- Expression and Crystallization of the Plant Alternative Oxidase