Title | DNA Replication [electronic resource] : Methods and Protocols / edited by Sonya Vengrova, Jacob Dalgaard |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2015 |
Edition | 2nd ed. 2015 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-2596-4 |
Descript | X, 295 p. 36 illus., 15 illus. in color. online resource |
A High-Throughput Confocal Fluorescence Microscopy Platform to Study DNA Replication Stress in Yeast Cells -- Microscopy Techniques to Examine DNA Replication in Fission Yeast -- High-Resolution Analysis of Mammalian DNA Replication Units -- Analysing the Dynamics of DNA Replication In Mammalian Cells Using DNA Combing -- Measuring DNA Content by Flow Cytometry in Fission Yeast -- Incorporation of Thymidine Analogs for Studying Replication Kinetics In Fission Yeast -- Edu Incorporation for FACS and Microscopy Analysis of DNA Replication in Budding Yeast -- Determination Of Deoxyribonucleoside Triphosphate Concentrations in Yeast Cells by Strong Anion-Exchange High-Performance Liquid Chromatography Coupled with Ultraviolet Detection -- Measuring Ribonucleotide Incorporation Into DNA In Vitro and In Vivo -- Detection And Sequencing Of Okazaki Fragments In S. Cerevisiae -- Chip-Seq To Analyze The Binding Of Replication Proteins To Chromatin -- Chromatin Immunoprecipitation To Detect DNA Replication And Repair Factors -- Molecular Genetic Methods To Study DNA Replication Protein Function In Haloferax Volcanii, A Model Archaeal Organism -- Single-Molecule Observation Of Prokaryotic DNA Replication -- Analysing the Response to Dysfunction Replication Forks Using the RTS1 Barrier System in Fission Yeast -- Purification of Restriction Fragments Containing Replication Intermediates from Complex Genomes for 2-D Gel Analysis -- Isolation of Restriction Fragments Containing Origins of Replication from Complex Genomes