Title | Chromatin Protocols [electronic resource] / edited by Srikumar P. Chellappan |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2015 |
Edition | 3rd ed. 2015 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-2474-5 |
Descript | XIV, 492 p. 74 illus., 36 illus. in color. online resource |
Beads-on-a-String on a Bead: Reconstitution and Analysis of Chromatin on a Solid Support -- Preparation and Analysis of Positioned Mononucleosomes -- Chromatin Imaging with Time-Lapse Atomic Force Microscopy -- Isolation of Specific Genomic Regions and Identification of Associated Molecules by Engineered DNA-Binding Molecule-Mediated Chromatin Immunoprecipitation (enChIP) Using CRISPR -- Drug-Induced Premature Chromosome Condensation (PCC) Protocols: Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin -- Analysis of Genomic Aberrations Using Comparative Genomic Hybridization of Metaphase Chromosomes -- Histone Deacetylase Activity Assay -- In Vitro Histone Demethylase Assays -- Integrated DNA Methylation and Chromatin Structural Analysis at Single-Molecule Resolution -- Determination of DNA Methylation Levels Using Illumina HumanMethylation450 BeadChips -- Investigation of Genomic Methylation Status Using Methylation Specific and Bisulfite Sequencing Polymerase Chain Reaction -- In Vitro and In Vivo Assays for Studying Histone Ubiquitination and Deubiquitination -- Immunostaining Analysis of Tissue Cultured Cells and Tissue Sections Using Phospho-Histone H3 (Serine 10) Antibody -- Identification and Characterization of Nonhistone Chromatin Proteins: Human Positive Coactivator 4 as a Candidate -- Methods to Study Transcription-Coupled Repair in Chromatin -- Analysis of DNA Replication Associated Chromatin Decondensation: In Vivo Assay for Understanding Chromatin Remodeling Mechanisms of Selected Proteins -- Non-Radioactive Assay Methods for the Assessment of Telomerase Activity and Telomere Length -- Detecting ATM Dependent Chromatin Modification in DNA Damage Response -- Imaging Local Deposition of Newly Synthesized Histones in UVC-Damaged Chromatin -- In Vitro Replication Assay with Mammalian Cell Extracts -- Fluorescent In Situ Hybridization on Comets: FISH Comet -- Methods to Study Histone Chaperone Function in Nucleosome Assembly and Chromatin Transcription -- Preparation of Mononucleosomal Templates for Analysis of Transcription with RNA Polymerase Using spFRET -- Transcriptome-Wide Identification of In Vivo Interactions Between RNAs and RNA-Binding Proteins by RIP and PAR-CLIP Assays -- Chromatin Immunoprecipitation Assays: Analyzing Transcription Factor Binding and Histone Modifications In Vivo -- ChIP on Chip and ChIP-Seq Assays: Genome-Wide Analysis of Transcription Factor Binding and Histone Modifications -- ChIP-on-Chip Analysis Methods for Affymetrix Tiling Arrays