Title | Affinity Chromatography [electronic resource] : Methods and Protocols / edited by Senta Reichelt |
---|---|
Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2015 |
Edition | 3rd ed. 2015 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-2447-9 |
Descript | XIII, 341 p. 78 illus., 37 illus. in color. online resource |
Affinity Chromatography: A Historical -- Protein Purification by Aminosquarylium Cyanine-Affinity Chromatography -- One Step Purification of Phosphinothricin Acetyltransferase using Reactive Dye Affinity Chromatography -- Antibody Purification from Human Plasma by Metal-Chelated Affinity Membranes -- Specific Recognition of Supercoiled Plasmid DNA by Affinity Chromatography using a Synthetic Aromatic Ligand -- Cell Affinity Separations on Microfluidic Devices -- Aptamer-modified :Magnetic Beads in Affinity Separation of Proteins -- The Strep-tag System for One-step Purification and High Affinity Detection or Capturing of Proteins -- Robotic High-throughput Purification of Affinity-tagged Recombinant Proteins -- Macroporous Silica Particles Derivatized for Enhanced Lectin Affinity Enrichment of Glycoproteins -- Immobilized Magnetic Beads based Multi-Target Affinity Selection Coupled with HPLC-MS for Screening Active Compounds from Traditional Chinese Medicine and Natural Products -- Mixed-bed Affinity Chromatography: Principles And Methods -- Preparation and characterization of fluorophenylboronic acid-functionalized Affinity Monolithic Columns for the Selective Enrichment of Cis-diol-containing Biomolecules -- Introduction to Macroporous Cryogels -- Cryogels with Affinity Ligands as Tools in Protein Purification -- Direct Capture of His6-tagged Proteins Using Megaporous Cryogels Developed for Metal-ion Affinity Chromatography -- Ni(II) Chelated IDA Functionalized Poly(HEMA-GMA) Cryogels for Urease Adsorption -- A Novel Chromatographic Media: Histidine Containing Composite Cryogels for HIgG Separation from Human Serum -- Molecularly Imprinted Cryogels for Human Serum Albumin Depletion -- Interpenetrating Polymer Network Composite Cryogels with Tailored Porous Morphology and Sorption Properties -- Analysis of Drug-Protein Interactions by High-Performance Affinity Chromatography: Interactions of Sulfonylurea Drugs with Normal and Glycated Human Serum Albumin -- Accurate protein-peptide Titration Experiments by Nuclear Magnetic Resonance using Low-volume Samples -- Characterization of the Binding Strengths between Boronic Acids and Cis-Diol-containing Biomolecules by Affinity Capillary Electrophoresis -- Determination of the Kinetic Rate Constant of Cyclodextrin Supramolecular Systems by High Performance Affinity Chromatography -- Molecular Modeling of the Affinity Chromatography of Monoclonal Antibodies