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TitleStem Cells and Good Manufacturing Practices [electronic resource] : Methods, Protocols, and Regulations
Author edited by Kursad Turksen
ImprintNew York, NY : Springer New York : Imprint: Humana Press, 2015
Connect tohttp://dx.doi.org/10.1007/978-1-4939-2435-6
Descript XI, 220 p. 34 illus. in color. online resource

SUMMARY

This volume collects a series of protocols describing the kinds of infrastructures, training, and standard operating procedures currently available to actualize the potential of stem cells for regenerative therapies. Stem Cells and Good Manufacturing Practices: Methods, Protocols, and Regulations pulls together key GMP techniques from laboratories around the world. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Inclusive and authoritative, Stem Cells and Good Manufacturing Practices: Methods, Protocols, and Regulations will be an invaluable resource to both basic and clinical practitioners in stem cell biology


CONTENT

Therapeutic Mesenchymal Stromal Cells: Where We Are Headed -- The Suspension Culture of Undifferentiated Human Pluripotent Stem Cells Using Spinner Flasks -- Scalable Expansion of Human Induced Pluripotent Stem Cells in Xeno-Free Microcarriers -- Derivation of GMP-Compliant Integration-Free hiPSCs Using Modified mRNAs -- Labeling Pluripotent Stem Cell-Derived Neural Progenitors with Iron Oxide Particles for Magnetic Resonance Imaging -- Using the Quantum Cell Expansion System for the Automated Expansion of Clinical-Grade Bone Marrow-Derived Human Mesenchymal Stromal Cells -- Isolation and Expansion of Mesenchymal Stromal/Stem Cells from Umbilical Cord Under Chemically Defined Conditions -- Production of Good Manufacturing Practice-Grade Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells for Therapeutic Use -- Xeno-Free Culture of Human Periodontal Ligament Stem Cells -- GMP-Compliant Human Adipose Tissue-Derived Stromal Cells for Cellular Therapy -- cGMP-Compliant Transportation Conditions for a Prompt Therapeutic Use of Marrow Mesenchymal Stromal/Stem Cells -- GMP-Grade Human Fetal Liver-Derived Mesenchymal Stem Cells for Clinical Transplantation -- Purification of Human Induced Pluripotent Stem Cell-Derived Neural Precursors Using Magnetic Activated Cell Sorting -- Scalable Ex Vivo Expansion of Human Mesenchymal Stem/Stromal Cells in Microcarrier-Based Stirred Culture Systems -- Media Fill for Validation of a Good Manufacturing Practice-Compliant Cell Production Process -- Standard Operating Procedure for the Good Manufacturing Practice-Compliant Production of Human Bone Marrow Mesenchymal Stem Cells -- Culture of Human Limbal Epithelial Stem Cells on Tenon’s Fibroblast Feeder-Layers: A Translational Approach -- Bioreactor Expansion of Human Mesenchymal Stem Cells According to GMP Requirements


Life sciences Cell biology Stem cells Life Sciences Cell Biology Stem Cells



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