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TitleNF-kappa B [electronic resource] : Methods and Protocols
Author edited by Michael J. May
ImprintNew York, NY : Springer New York : Imprint: Humana Press, 2015
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Descript XVI, 668 p. 117 illus., 61 illus. in color. online resource


Focusing on essential insight into the methods and techniques required to dissect the complex mechanisms of NF-κB activation, regulation, and function. NF-kappa B: Methods and Protocols guides readers through standard approaches to detect NF-κB pathway activation, detection and analysis of NF-κB signaling, and methods to study the control of NF-κB signaling. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.   Comprehensive and authoritative, NF-Kappa B: Methods and Protocols provides a timely and invaluable resource for researchers seeking to perform experiments aimed at understanding the role of NF-κB signaling in health and disease


Electrophoretic Mobility Shift Assay Analysis of NF-B DNA Binding -- Detection of IkB Degradation Dynamics and IkB-a Ubiquitination -- Measurement of NF-κB Transcriptional Activity and Identification of NF-κB cis-regulatory Elements using Luciferase Assays -- Assessing Sites of NF-κB DNA Binding using Chromatin Immunoprecipitation -- IKK Kinase Assay for Assessment of Canonical NF-kB Activation in Neurons -- Analysis of Epidermal Growth Factor-induced NF-kB Signaling -- Methods to Assess the Activation of the Alternative (non-canonical) NF-kB Pathway by Non-death TNF Receptors -- Systematic Detection of Non-canonical NF-κB Activation -- Non-canonical NF-κB activation and SDF-1 expression in Human Endothelial Cells -- Stable Reconstitution of IKK-deficient Mouse Embryonic Fibroblasts -- Dissecting NF-κB Signaling Induced by Genotoxic Agents Via Genetic Complementation of NEMO-deficient 1.3E2 Cells -- Visualizing TCR induced POLKADOTS Formation and NF-κB Activation in the D10 T Cell Clone and Mouse Primary Effector T cells -- Detection of Recombinant and Cellular MALT1 Paracaspase Activity -- TRAF Protein Function In Non-Canonical NF-κB Signaling -- Roles of c-IAP Proteins in TNF Receptor Family Activation of NF-kB Signaling -- Elucidating Dynamic Protein-protein Interactions and Ubiquitination in NF-kB Signaling Pathways -- Immunoblot Analysis of Linear Polyubiquitination of NEMO -- In vitro Detection of NEMO-Ubiquitin Binding using DELFIA and Microscale Thermophoresis Assays -- Use of Fluorescence Spectroscopy For Quantitative Investigations Of Ubiquitin Interactions with the Ubiquitin-Binding Domains of NEMO -- Generation of a Proteolytic Signal: E3/E2-mediated Polyubiquitination of IκBα -- Control of NF-κB Subunits by Ubiquitination -- Methodology to Study NF-kB/RelA Ubiquitination in vivo -- Using Sequential Immunoprecipitation and Mass Spectrometry to Identify Methylation of NF-kB -- Methods to Detect NF-kB Acetylation and Methylation -- A Method for the Quantitative Analysis of Stimulation-Induced Nuclear Translocation of the p65 subunit of NF-kB from Patient-Derived Dermal Fibroblasts -- Methods for Assessing the In Vitro Transforming Activity of NF-κB Transcription Factor c-Rel and Related Proteins -- Using RNA Interference in Lung Cancer Cells to Target the IKK-NF-κB Pathway -- Immunohistochemical Analysis of NF-kB in Human Tumor Tissue -- Assessment of Canonical NF-kB Activity in Canine Diffuse Large B-Cell Lymphoma -- NEMO Binding Domain Peptide Inhibition of Inflammatory Signal-Induced NF-κB Activation In Vivo -- Regulation of NFκB Signaling in Osteoclasts and Myeloid Progenitors -- NF-κB Activation with Aging:  Characterization and Therapeutic Inhibition -- The “Sneaking-ligand” Approach: Cell-type Specific Inhibition of the Classical NF-kB Pathway -- Sneaking-ligand Fusion Proteins Attenuate Serum Transfer Arthritis by Endothelium-targeted NF-kB inhibition -- Analysis of NF-kB Activation in Mouse Intestinal Epithelial Cells -- Characterizing the DNA Binding Site Specificity of NF-kB with Protein Binding Microarrays (PBMs) -- Methods for Analyzing the Evolutionary Relationship of NF-kB Proteins Using Free, Web-driven Bioinformatics and Phylogenetic Tools -- Studying NFkB Signaling with Mathematical Models

Life sciences Biochemistry Posttranslational modification Life Sciences Biochemistry general Posttranslational Modification


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