Title | Insoluble Proteins [electronic resource] : Methods and Protocols / edited by Elena García-Fruitós |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2015 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-2205-5 |
Descript | XVI, 425 p. 72 illus., 41 illus. in color. online resource |
General Introduction: Recombinant Protein Production and Purification of Insoluble Proteins -- Overcoming the Solubility Problem in E. coli: Available Approaches for Recombinant Protein Production -- Optimization of Culture Parameters and Novel Strategies to Improve Protein Solubility -- Cleavable Self-Aggregating Tags (cSAT) for Protein Expression and Purification -- Beyond the Cytoplasm of Escherichia coli: Localizing Recombinant Proteins Where You Want Them -- Characterization of Amyloid-Like Properties in Bacterial Intracellular Aggregates -- Co-Translational Stabilization of Insoluble Proteins in Cell-Free Expression Systems -- Functional Expression of Plant Membrane Proteins in Lactococcus lactis -- High Cell-Density Expression System: Yeast Cells in a Phalanx Efficiently Produce a Certain Range of “Difficult-to-Express” Secretory Recombinant Proteins -- Insect Cells-Baculovirus System for the Production of Difficult to Express Proteins -- Transient Expression in HEK 293 Cells: An Alternative to E. coli for the Production of Secreted and Intracellular Mammalian Proteins -- Recombinant Glycoprotein Production in Human Cell Lines -- Soluble Recombinant Protein Production in Pseudoaltermonas haloplanktis TAC125 -- A Screening Methodology for Purifying Proteins with Aggregation Problems -- Solubilization and Refolding of Inclusion Body Proteins -- Bacterial Inclusion Body Purification -- Characterization of Intracellular Aggresomes by Fluorescent Microscopy -- Dialysis: A Characterization Method of Aggregation Tendency -- Applications of Mass Spectrometry to the Study of Protein Aggregation -- Insoluble Protein Assemblies Characterized by Fourier Transform Infrared Spectroscopy -- Insoluble Protein Characterization by Circular Dichroism (CD) Spectroscopy and Nuclear Magnetic Resonance (NMR) -- Methods for Characterization of Protein Aggregates -- Predicting the Solubility of Recombinant Proteins in Escherichia coli -- Insoluble Protein Applications: The Use of Bacterial Inclusion Bodies as Biocatalysts