Title | Polyadenylation in Plants [electronic resource] : Methods and Protocols / edited by Arthur G. Hunt, Qingshun Quinn Li |
---|---|
Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2015 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-2175-1 |
Descript | IX, 225 p. 34 illus., 23 illus. in color. online resource |
Computational Analysis Of Plant Polyadenylation Signals -- Prediction of Plant mRNA Polyadenylation Sites -- Extraction of poly(A) Sites from Large-scale RNA-seq Data -- Poly(A)-tag Deep Sequencing Data Processing To Extract poly(A) Sites -- Analysis of poly(A) Site Choice Using A Java-Based Clustering Algorithm -- RADPRE: a Computational Program for Identification of Differential mRNA Processing Including Alternative Polyadenylation -- Characterization Of Plant Polyadenylation Complexes By Using Tandem Affinity Purification -- In vitro Analysis Of Cleavage And Polyadenylation In Arabidopsis -- Production, Purification, and Assay of Recombinant Proteins for in vitro Biochemical Analyses of the Plant Polyadenylation Complex -- Detection of Disulfide Linkage By Chemical Derivatization And Mass Spectrometry -- Transient Expression Using Agroinfiltration To Study Polyadenylation In Plants -- A 3’ RACE Protocol To Confirm Polyadenylation Sites -- Phage Display Library Screening for Identification of Interacting Protein Partners -- Genome-wide Determination Of Poly(A) Site Choice In Plants -- DNA/RNA Hybrid Primer Mediated Poly(A) Tag Library Construction for Illumina Sequencing -- Poly(A) Tag Library Construction from 10 ng Total RNA -- Genome-Wide Analysis Of Distribution of RNA Polymerase II isoforms using ChIP-seq