XII, 278 p. 52 illus., 47 illus. in color. online resource
SUMMARY
This volume seeks to familiarize readers with a diverse range of technologies and approaches for probing neuron and circuit architecture, and, when possible, to attach detailed protocols to help guide readers toward practical application. From classical lipophilic dye and conjugated lectin tracing techniques, to electrophysiological, in vivo imaging, viral tract tracing, and emerging genetic methods to mark, manipulate, and monitor neural circuits, Neural Tracing Methods: Tracing Neurons and Their Connections includes reference to an arsenal of tools and technologies currently being implemented in model systems ranging from flies to mice. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Essential and authoritative, Neural Tracing Methods: Tracing Neurons and Their Connections collects a comprehensive compilation of chapters authored by inventors and expert users, that describes state-of-the-art neuronal tracing and functional analysis methods in order to aid researchers in continuing this vital pathway of study
CONTENT
A Survey of Current Neuroanatomical Tracing Techniques.- Wheat-Germ Agglutinin (WGA) Tracing: A Classic Approach for Unraveling Neural Circuitry -- Retrograde Tract-Tracing ‘Plus’: Adding Extra Value to Retrogradely-Traced Neurons -- Simultaneous Collection of In Vivo Functional and Anatomical Data from Individual Neurons in Awake Mice -- Single-Cell Electroporation for In Vivo Imaging of Neuronal Morphology and Growth Dynamics -- Practical Methods for In Vivo Cortical Physiology with 2-Photon Microscopy and Bulk Loading of Fluorescent Calcium Indicator Dyes -- Optogenetic Dissection of Neural Circuit Function in Behaving Animals -- Remote Control of Neural Activity Using Chemical Genetics -- Generation of BAC Transgenic Mice for Functional Analysis of Neural Circuits -- Engineered Rabies Virus for Transsynaptic Circuit Tracing -- Genetic Labeling of Synapses -- Genetic Pathways to Circuit Understanding in Drosophila
