Title | Bacterial Pangenomics [electronic resource] : Methods and Protocols / edited by Alessio Mengoni, Marco Galardini, Marco Fondi |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2015 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-1720-4 |
Descript | X, 298 p. 59 illus., 42 illus. in color. online resource |
Pulsed Field Gel Electrophoresis and Genome Size Estimates -- Comparative Analyses of Extrachromosomal Bacterial Replicons, Identification of Chromids and Experimental Evaluation of their Indispensability -- Choice of Next Generation Sequencin Pipelines -- The Pyrosequencing Protocol for Bacterial Genomes -- Bacterial Metabarcoding by 16S rRNA Gene Ion Torrent Amplicon Sequencing -- The Ilumina-Solexa Sequencing Protocol for Bacterial Genomes -- High-Throughput Phenomics -- Comparative Analysis of Gene Expression: Uncovering Expression Conservation and Divergence between Salmonella enterica serovar Typhimurium strains LT2 and 14028S -- Raw Sequence Data and Quality Control -- Methods for Assembling Reads and Producing Contigs -- Mapping Contigs Using CONTIGuator -- Gene Calling and Bacterial Genome Annotation with BG7 -- Defining Orthologs and Pangenome Size Metrics -- Robust Identification of Orthologues and Paralogues for Microbial Pan-Genomics Using GET_HOMOLOGUES: A Case Study of pIncA/C Plasmids -- Genome-Scale Metabolic Networks Reconstruction -- From Pangenome to Phanphenome and Back -- Genome-Wide Detection of Selection and Other Evolutionary Forces -- The Integrated Microbial Genome Resource of Analysis