Title | The Nucleus [electronic resource] / edited by Ronald Hancock |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2015 |
Edition | 2nd ed. 2015 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-1680-1 |
Descript | XI, 237 p. 54 illus., 35 illus. in color. online resource |
Cell Type-Specific Affinity Purification of Nuclei for Chromatin Profiling in Whole Animals -- Lysis Gradient Centrifugation – A Flexible Method for the Isolation of Nuclei from Primary Cells -- Isolation of Nuclei in Media Containing an Inert Polymer to Mimic the Crowded Cytoplasm -- A New Rapid Method for Isolating Nucleoli -- Sequential Recovery of Macromolecular Components of the Nucleolus -- Au Nanoinjectors for Electrotriggered Gene Delivery into the Cell Nucleus -- Improving Chromatin Immunoprecipitation (ChIP) by Suppression of Method-Induced DNA-Damage Signaling -- Purification of Specific Chromatin Loci for Proteomic Analysis -- Chromatin Structure Analysis of Single Gene Molecules by Psoralen Crosslinking and Electron Microscopy -- Purification of Proteins on Newly Synthesized DNA Using iPOND -- Applying the Ribopuromycylation Method to Detect Nuclear Translation -- Targeted Nano Analysis of Water and Ions in the Nucleus Using Cryo-Correlative Microscopy -- A Redox-Sensitive Yellow Fluorescent Protein Sensor for Monitoring Nuclear Glutathione Redox Dynamics -- Determination of the Dissociation Constant of the NFkB p50/p65 Heterodimer in Living Cells Using Fluorescence Cross-Correlation Spectroscopy -- Imaging and Quantification of Amyloid Fibrillation in the Cell Nucleus -- Analysis of Nuclear Organization with TANGO, Software for High-Throughput Quantitative Analysis of 3D Fluorescence Microscopy Images -- Quantitative Analysis of Chromosome Localization in the Nucleus