Title | Bacterial Artificial Chromosomes [electronic resource] / edited by Kumaran Narayanan |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2015 |
Edition | 2nd ed. 2015 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-1652-8 |
Descript | XI, 348 p. 48 illus., 36 illus. in color. online resource |
From Selective Full-Length Genes Isolation By TAR Cloning In Yeast To Their Expression From HAC Vectors In Human Cells -- Recombineering Linear BACs -- BAC Sequencing Using Pooled Methods -- Making BAC Transgene Constructs With Lambda-Red Recombineering System For Transgenic Animals Or Cell Lines -- Directing Enhancer-Traps And Itol2 End Sequences To Deleted BAC Ends With Loxp- and Lox511-Tn10 Transposons -- Recombining Overlapping Bacs Into Single Large Bacs -- Generation Of Knockout Alleles By RFLP Based BAC Targeting Of Polymorphic Embryonic Stem Cells -- Herpesvirus Mutagenesis Facilitated By Infectious Bacterial Artificial Chromosomes (Ibacs) -- Conversion Of BAC Clones Into Binary BAC (BIBAC) Vectors and Their Delivery Into Basidiomycete Fungal Cells Using Agrobacterium tumefaciens -- Infectious Delivery of Alphaherpesvirus Bacterial Artificial Chromosomes -- A Recombineering-Based Gene Tagging System For Arabidopsis -- BAC Transgenic Zebrafish for Transcriptional Promoter and Enhancer Studies -- Bacs-On-BeadsTM (BobsTM) Assay for the Genetic Evaluation of Prenatal Samples And Products of Conception -- New BAC Probe Set To Narrow Down Chromosomal Breakpoints In Small and Large Derivative Chromosomes, Especially Suited for Mosaic Conditions -- BAC-Probes Applied For Characterization Of Fragile Sites (FS) -- Application Of BAC-Probes To Visualize Copy Number Variants (CNVs) -- Site-Specific Integration Of Bacterial Artificial Chromosomes Into Human Cells -- Generation Of BAC Reporter Cell Lines For Drug Discovery