Title | Plant Endosomes [electronic resource] : Methods and Protocols / edited by Marisa S. Otegui |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2014 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-1420-3 |
Descript | XI, 286 p. 51 illus., 30 illus. in color. online resource |
The Use of FM Dyes to Analyze Plant Endocytosis -- Sterol Dynamics During Endocytic Trafficking in Arabidopsis -- Live Microscopy Analysis of Endosomes and Vesicles in Tip-Growing Root Hairs -- Analysis of Fluid Phase Endocytosis in (Intact) Plant Cells -- Immunogold Labeling and Electron Tomography of Plant Endosomes -- Investigating Protein-Protein Interactions in the Plant Endomembrane System Using Multiphoton-Induced FRET-FLIM -- Analysis of Rab GTPase-Effector Interactions by Bimolecular Fluorescence Complementation -- In Vivo Imaging of Brassinosteroid Endocytosis in Arabidopsis -- Analysis of Prevacuolar Compartment-Mediated Vacuolar Proteins Transport -- Evaluation of Defective Endosomal Trafficking to the Vacuole by Monitoring Seed Storage Proteins in Arabidopsis thaliana -- Trans-Species Complementation Analysis to Study Function Conservation of Plant Endosomal Sorting Complex Required for Transport (ESCRT) Proteins -- A Re-Elicitation Assay to Correlate flg22-Signaling Competency with Ligand-Induced Endocytic Degradation of the FLS2 Receptor -- Preparation of Enriched Plant Clathrin-Coated Vesicles by Differential and Density Gradient Centrifugation -- Proteomics of Endosomal Compartments from Plants Case Study: Isolation of trans-Golgi Network Vesicles -- Analysis of Global Ubiquitylation and Ubiquitin-Binding Domains Involved in Endosomal Trafficking -- Analysis of Endocytosis and Ubiquitination of the BOR1 Transporter -- Ubiquitination of Plant Immune Receptors -- Ubiquitylation-Mediated Control of Polar Auxin Transport: Analysis of Arabidopsis PIN2 Auxin Transport Protein -- Chemical Genomics Screening for Biomodulators of Endomembrane System Trafficking -- Integrative Chemical Proteomics and Cell Biology Methods to Study Endocytosis and Vesicular Trafficking in Arabidopsis