Title | Regulatory Non-Coding RNAs [electronic resource] : Methods and Protocols / edited by Gordon G. Carmichael |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2015 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-1369-5 |
Descript | X, 181 p. 39 illus., 10 illus. in color. online resource |
Diverse Functions and Mechanisms of Mammalian Long Non-Coding RNAs -- Sequence-Defined Oligoaminoamides for the Delivery of siRNAs -- A Biochemical Approach to Identify Direct MicroRNA Targets -- Native Gel Analysis for Mammalian MicroRNPs Assembled from Pre-MicroRNAs -- Identification and Validation of miRNA Target Sites within Non-Traditional miRNA Targets -- Fractionation of Non-Polyadenylated and Ribosomal-Free RNAs from Mammalian Cells -- Affinity-Purification of Long Noncoding RNA−Protein Complexes from Formaldehyde-Cross linked Mammalian Cells -- RNA Pull down Protocol for In Vitro Detection and Identification of RNA-Associated Proteins -- nRIP-seq: A Technique to Identify RNA Targets of an RNA Binding Protein on a Genome-Wide Scale -- Analysis of the Subcellular Distribution of RNA by Fluorescence In Situ Hybridization -- RNA Fluorescence In Situ Hybridization in Cultured Mammalian Cells -- Locating RNAs In Situ with FISH-STIC Probes -- Rapid and Efficient Elimination of Specific Nuclear Noncoding RNAs in Mammalian Cells with Antisense Oligonucleotides -- On Using Magnesium and Potassium Ions in RNA Experiments -- Studying the Affinity, Kinetic Stability and Specificity of RNA/Protein Interactions: SINE ncRNA/Pol II Complexes as a Model System