Title | Hematopoietic Stem Cell Protocols [electronic resource] / edited by Kevin D. Bunting, Cheng-Kui Qu |
---|---|
Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2014 |
Edition | 3rd ed. 2014 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-1133-2 |
Descript | XII, 364 p. 52 illus., 42 illus. in color. online resource |
The Hematopoietic Stem Cell Landscape -- Investigating the Interaction Between Hematopoietic Stem Cells and Their Niche During Embryonic Development: Optimizing the Isolation of Fetal and Newborn Stem Cells From Liver, Spleen, and Bone Marrow -- Single-Cell PCR Profiling of Gene Expression in Hematopoiesis -- Hematopoietic Stem and Progenitor Cell Mobilization in Mice -- Cell Cycle Measurement of Mouse Hematopoietic Stem/Progenitor Cells -- Flow Cytometry Analysis of Signaling and Apoptosis in Hematopoietic Stem Cells -- Gene Expression Profiling of Hematopoietic Stem Cells (HSC) -- Measuring MicroRNA Expression in Mouse Hematopoietic Stem Cells -- DNA Methylation Profiling of Hematopoietic Stem Cells -- Metabolic Characterization of Hematopoietic Stem Cells -- Nano Proteomic Assays on Hematopoietic Stem Cells -- Hematopoietic Differentiation of Pluripotent Stem Cells in Culture -- Ex vivo Assays to Study Self-Renewal, Long-Term Expansion and Leukemic Transformation of Genetically Modified Human Hematopoietic and Patient-Derived Leukemic Stem Cells -- Ex vivo Expansion of Murine and Human Hematopoietic Stem Cells -- Using Microfluidics to Investigate Hematopoietic Stem Cell and Micro niche Interactions at the Single Cell Level -- Five Lineage Clonal Analysis of Hematopoietic Stem/Progenitor Cells -- Intravital Imaging of Hematopoietic Stem Cells in the Mouse Skull -- Immune Deficient Mouse Model for Human Hematopoietic Stem Cell Engraftment and Immune System Development -- Homing and Migration Assays of Hematopoietic Stem/Progenitor Cells -- Retroviral Transduction of Murine and Human Hematopoietic Progenitors and Stem Cells -- Lentiviral Gene Transduction of Mouse and Human Hematopoietic Stem Cells -- High Throughput Genomic Mapping of Vector Integration Sites in Gene Therapy Studies -- Barcoded Vector Libraries and Retro- or Lentiviral Barcoding of Hematopoietic Stem Cells -- Barcoded Vector Libraries and Retro- or Lentiviiral Barcoding of Hematopoietic Stem Cells -- Using Microfluidics to Investigate Hematopoietic Stem Cell and Micro niche Interactions at the Single Cell Level -- Five Lineage Clonal Analysis of Hematopoietic Stem/Progenitor Cells -- Intravital Imaging of Hematopoietic Stem Cells in the Mouse Skull -- Immune Deficient Mouse Model for Human Hematopoietic Stem Cell Engraftment and Immune System Development -- Homing and Migration Assays of Hematopoietic Stem/Progenitor Cells -- Retroviral Transduction of Murine and Human Hematopoietic Progenitors and Stem Cells -- Lentiviral Gene Transduction of Mouse and Human Hematopoietic Stem Cells -- High Throughput Genomic Mapping of Vector Integration Sites in Gene Therapy Studies -- Barcoded Vector Libraries and Retro- or Lentiviiral Barcoding of Hematopoietic Stem Cells