Title | Exocytosis and Endocytosis [electronic resource] / edited by Andrei I. Ivanov |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2014 |
Edition | 2nd ed. 2014 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-0944-5 |
Descript | XIII, 435 p. 93 illus., 55 illus. in color. online resource |
Pharmacological Inhibitors of Exocytosis and Endocytosis: Novel Bullets for Old Targets -- Systematic Analysis of Endocytosis by Cellular Perturbations -- Real Time Detection of SNARE Complexes with FRET Using the Tetracysteine System -- Profiling Lysine Ubiquitination by Selective Enrichment of Ubiquitin Remnant-Containing Peptides -- Secretion of Circular Proteins Using Sortase -- Fractionation of Subcellular Membrane Vesicles of Epithelial and Non-Epithelial Cells by OptiPrepTM Density Gradient Ultracentrifugation -- Combining Pulsed SILAC Labeling and Click-Chemistry for Quantitative Secretome Analysis -- Probabilistic Density Maps to Study the Spatial Organization of Endocytosis -- Use of Kaede and Kikume Green-Red Fusions for Live Cell Imaging of G Protein-Coupled Receptors -- Halo Tag as a Tool to Investigate Peroxisome Dynamics in Cultured Mammalian Cells -- SNAP-Tag to Monitor Trafficking of Membrane Proteins in Polarized Epithelial Cells -- FLAsH-PALM: Super-Resolution Pointillist Imaging with FLAsH-tetracysteine Labeling -- Analysis of Protein Dynamics with Tandem Fluorescent Protein Timers -- Synchronization of Secretory Cargo Trafficking in Populations of Cells -- Use of Transmembrane FRET to Investigate the Internalization of Glycosylated Proteins -- A Method to Rapidly Induce Organelle-specific Molecular Activities and Membrane Tethering -- A Novel Pair of Split Venus Fragments to Detect Protein-Protein Interactions by In Vitro and In Vivo Biomolecular Fluorescence Complementation Assays -- Real Time Investigation of Plasma Membrane Deformation and Fusion Pore Expansion Using Polarized Total Internal Reflection Fluorescence Microscopy -- Nanocones to Study Initial Steps of Endocytosis -- A Novel Permeabilization Protocol to Obtain Intracellular 3D Immunolabeling for Electron Tomography -- VIS2FIX: Rapid Chemical Fixation of Vitreous Sections for Immuno-Electron Microscopy -- Chemical Genomics: Characterizing Target Pathways for Bioactive Compounds Using the Endomembrane Trafficking Network -- Application of RNAi Technology and Fluorescent Protein Markers to Study Membrane Traffic in C. elegans -- Visualization of Clathrin-Mediated Endocytosis in Live Drosophila Egg Chambers -- Novel Extraction Protocol to Probe the Role of Cholesterol in Synaptic Vesicle Recycling -- Microfluidic Devices for Imaging Trafficking Events In Vivo Using Genetic Model Organisms -- The “In Situ” Proximity Ligation Assay to Probe Protein-Protein Interactions in Intact Tissues -- Probing the Role of the Actin Cytoskeleton During Regulated Exocytosis by Intravital Microscopy -- Measurement of Dynamic F-actin Changes During Exocytosis