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TitleCancer Cell Signaling [electronic resource] : Methods and Protocols
Author edited by Martha Robles-Flores
ImprintNew York, NY : Springer New York : Imprint: Humana Press, 2014
Edition 2nd ed. 2014
Connect tohttp://dx.doi.org/10.1007/978-1-4939-0856-1
Descript XI, 263 p. 42 illus., 27 illus. in color. online resource

SUMMARY

Cancer Cell Signaling: Methods and Protocols, Second Edition brings together the most recent advances in cancer cell signaling knowledge along with recent progress in the development of novel strategies and experimental cell models to study human cancer. Opening with a section on targeting cancer cell metabolism as an anticancer strategy, this detailed volume continues with parts describing protocols to study epigenetic control of cancer, protocols to study cancer metastasis, as well as two new techniques for cancer study.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions for their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and timely, Cancer Cell Signaling: Methods and Protocols, Second Edition is an ideal guide to the techniques that will aid researchers in exploiting our current knowledge in order to make a profound impact in cancer prevention and treatment


CONTENT

Use of Biguanides to Improve Response to Chemotherapy -- Evaluating Response to Metformin/Cisplatin Combination in Cancer Cells Via Metabolic Measurement and Clonogenic Survival -- Quantifying the Autophagy-Triggering Effects of Drugs in Cell Spheroids with Live Fluorescence Microscopy -- Interdependency Between Genetic and Epigenetic Regulatory Defects in Cancer -- Experimental Strategies to Manipulate the Cellular Levels of the Multifunctional Factor CTCF -- Use of Serum-Circulating miRNA Profiling for the Identification of Breast Cancer Biomarkers -- Prostate Cancer Detection Using a Non-Invasive Method for Quantifying mi RNAs -- DNA Methylation Analysis of Steroid Hormone Receptor Genes -- Control of Oncogenic mi RNA Function by Light-Activated mi RNA Antagomirs -- Methods for the Study of Long Non-Coding RNA in Cancer Cell Signaling -- Microvesicles as Mediators of Intercellular Communication in Cancer -- In Vivo Rat Model to Study Horizontal Tumor Progression -- An In Vivo Model to Study the Effects of Tumoral Soluble Factors on the Vascular Permeability in Mice -- Use of the Tumor Repressor DEDD as a Prognostic Marker of Cancer Metastasis -- Zebrafish-Based Systems Pharmacology of Cancer Metastasis -- A Flow Cytometry-Based Assay for the Evaluation of Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) in Cancer Cells -- Gene Disruption Using Zinc Finger Nuclease Technology


Medicine Cancer research Biomedicine Cancer Research



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