Title | Plant Isoprenoids [electronic resource] : Methods and Protocols / edited by Manuel Rodríguez-Concepción |
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Imprint | New York, NY : Springer New York : Imprint: Humana Press, 2014 |
Connect to | http://dx.doi.org/10.1007/978-1-4939-0606-2 |
Descript | XI, 303 p. 53 illus., 12 illus. in color. online resource |
Plant Isoprenoids: A General Overview -- Measuring the Activity of 1-Deoxy-D-Xylulose 5-Phosphate Synthase, the First Enzyme in the MEP Pathway, in Plant Extracts -- Determination of 3-Hydroxy-3-methylglutaryl CoA Reductase Activity in Plants -- Farnesyl Diphosphate Synthase Assay -- Metabolite Profiling of Plastidial Deoxyxylulose-5-Phosphate Pathway Intermediates by Liquid Chromatography and Mass Spectrometry -- Analysis of Carotenoids and Tocopherols in Plant Matrices and Assessment of Their In Vitro Antioxidant Capacity -- Simultaneous Analyses of Oxidized and Reduced Forms of Photosynthetic Quinones by High-Performance Liquid Chromatography -- Determination of Sterol Lipids in Plant Tissues by Gas Chromatography and Q-TOF Mass Spectrometry -- Analysis of Plant Polyisoprenoids -- Analysis of Diterpenes and Triterpenes from Plant Foliage and Roots -- Gas Chromatography Mass-Spectrometry Method for Determination of Biogenic Volatile Organic Compounds Emitted by Plants -- Analysis of Steroidal Alkaloids and Saponins in Solanaceae Plant Extracts using UPLC-qTOF Mass Spectrometry -- Isoprenoid and Metabolite Profiling of Plant Trichomes -- Sample Preparation for Single Cell Transcriptomics - Essential Oil Glands in Citrus Fruit Peel as an Example -- Prenylquinone Profiling in Whole Leaves and Chloroplast Subfractions -- Confocal Laser Scanning Microscopy Detection of Chlorophylls and Carotenoids in Chloroplasts and Chromoplasts of Tomato Fruit -- Heterologous Expression of Triterpene Biosynthetic Genes in Yeast and Subsequent Metabolite Identification Through GC-MS -- High Throughput Testing of Terpenoid Biosynthesis Candidate Genes Using Transient Expression in Nicotiana benthamiana -- Heterologous Stable Expression of Terpenoid Biosynthetic Genes Using the Moss Physcomitrella patens -- Quantification of Plant Resistance to Isoprenoid Biosynthesis Inhibitors -- A Flexible Protocol for Targeted Gene Co-Expression Network Analysis