Author	Heida, Tjitske. author
Title	Electric Field-Induced Effects on Neuronal Cell Biology Accompanying Dielectrophoretic Trapping [electronic resource] / by Tjitske Heida
Imprint	Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 2003
Connect to	http://dx.doi.org/10.1007/978-3-642-55469-8
Descript	IX, 80p. 46 illus. online resource

The concept of the cultured neuron probe was induced by the possible selective stimulation of nerves for functional recovery after a neural lesion or disease. The probe consists of a micro-electrode array on top of which groups of neuronal cells are cultured. An efficient method to position groups of neuronal cells on top of the stimulation sites of the micro-electrode array is developed. With negative dielectrophoretic forces, produced by non-uniform electric fields on polarizable particles, neuronal cells are trapped. Experimental results and model simulations describe the trapping process and its effect on neuronal cell viability.

1 Introduction -- 1.1 Neuro-Electronic Interfacing -- 1.2 Culturing Neuronal Cells -- 1.3 Positioning and Culturing Neuronal Cells on a Microelectrode Array -- 1.4 Dielectrophoresis -- 1.5 Scope of This Review -- 2 Dielectrophoretic Trapping of Neuronal Cells -- 2.1 Theory -- 2.2 Materials -- 2.3 Theoretical Description of Dielectrophoretic Trapping -- 2.4 Experimental Description of Dielectrophoretic Trapping -- 3 Exposing Neuronal Cells to Electric Fields -- 3.1 Theory -- 3.2 Theoretical Investigation of Induced Membrane Potentials of Neuronal Cells -- 3.3 Experimental Investigation of Neuronal Membrane Breakdown -- 4 Investigating Viability of Dielectrophoretically Trapped Neuronal Cells -- 4.1 Viability of Neuronal Cells Trapped at a High Frequency -- 4.2 Viability of Neuronal Cells Trapped at Low Frequencies -- 4.3 Recording Neuronal Activity -- 5 Summary -- References