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TitleClinical and Forensic Applications of Capillary Electrophoresis [electronic resource] / edited by John R. Petersen, Amin A. Mohammad
ImprintTotowa, NJ : Humana Press : Imprint: Humana Press, 2001
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Descript X, 454 p. online resource


Because capillary electrophoresis (CE) is driving the automation of manual and semi-manual techniques, conserves precious samples, and minimizes the use of hazardous organic chemicals, it constitutes a powerful new methodology for the clinical laboratory. In Clinical and Forensic Applications of Capillary Electrophoresis, John R. Petersen and Amin A. Mohammad, along with a panel of leading basic and clinical investigators, review those CE methods that are now replacing many routine serum and blood tests in clinical and forensic laboratories. Major areas reviewed include the coating of columns; the analysis of serum, urine, and CSF proteins and paraproteins; abnormal hemoglobins and hemoglobin Alc; peptides, amino and organic acids; therapeutic drugs; drugs of abuse; viral load; and short tandem repeats (STR). The methods discussed include capillary zone, micellar, electrokinetic, capillary gel, and nonaqueous electrophoresis. The authors show how a variety of detection methods (UV-visible, fluorescence, and MS) can be used in conjunction with CE to detect a broad array of analytes, ranging from serum proteins to PCR products to drugs of forensic importance. Innovative and highly practical, Clinical and Forensic Applications of Capillary Electrophoresis demonstrates the power and versatility of CE-not only to develop new assays, but also to markedly simplify today's clinical and forensic laboratory methodology


1 Capillary Electrophoresis: General Overview and Applications in the Clinical Laboratory -- 2 Basic Principles and Modes of Capillary Electrophoresis -- 3 Capillary Coatings for Protein Analysis -- 4 Clinical Serum Protein Capillary Zone Electrophoresis -- 5 Serum and Urine Paraprotein Capillary Electrophoresis -- 6 Cerebrospinal Fluid Protein Electrophoresis -- 7 Lipoprotein Analysis -- 8 Clinical Analysis of Structural Hemoglobin Variants and Hb A1c by Capillary Isoelectric Focusing -- 9 Amino Acid Analysis -- 10 Organic Acids -- 11 Steroids -- 12 Capillary Electrophoresis Based Immunoassay -- 13 Quantitation of Viral Load -- 14 The Application of Capillary Electrophoresis in the Analysis of PCR Products Used in Forensic DNA Typing -- 15 Combining Capillary Electrophoresis with Electrospray Ionization Mass Spectrometry -- 16 Capillary Electrophoresis-Mass Spectrometry of Biologically Active Peptides and Proteins -- 17 Serum Drug Monitoring by Capillary Electrophoresis -- 18 Applications of Capillary Zone Electrophoresis in the Analysis of Metal Ions of Clinical Significance -- 19 Clinical and Forensic Drug Toxicology: Analysis of Illicit and Abused Drugs in Urine by Capillary Electrophoresis -- 20 Screening Biological Specimens for Drugs of Forensic Significance

Medicine Pathology Medicine & Public Health Pathology


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