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TitleMolecular Diagnostics [electronic resource] : For the Clinical Laboratorian / edited by William B. Coleman, Gregory J. Tsongalis
ImprintTotowa, NJ : Humana Press : Imprint: Humana Press, 1997
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Descript X, 390 p. online resource


In Molecular Diagnostics For the Clinical Laboratorian notable practitioners describe how laboratory medicine is practiced today and illuminate how it will function tomorrow as the revolutionary advances afforded by molecular diagnostics become increasingly central to effective analysis. Proceeding from a discussion of elementary nucleic acid technology to a review of the more advanced techniques, the distinguished contributors lay the groundwork for a comprehensive understanding of their applications throughout clinical medicine. The result is a detailed description of those molecular technologies currently used in diagnostic laboratories, as well as those that seem particularly promising. Detailed discussions of specific clinical applications include those for cancer, hematological malignancies, cardiovascular disease, and neuromuscular, endocrine, and infectious diseases. Molecular Diagnostics For the Clinical Laboratorian captures for active physicians and clinical laboratory scientists a practical sense of the rapidly evolving applications of molecular biology now being introduced to the medical community at record speeds. Presenting this powerful new technology in a concise, readily understandable fashion, this book constitutes the premier tutorial guide to today's molecular diagnostic techniques


I Basic Molecular Biology -- 1 A Historical Perspective on the Clinical Diagnostic Laboratory -- 2 An Overview of Nucleic Acid Chemistry, Structure, and Function: The Foundations of Molecular Biology -- 3 Basic Nucleic Acid Procedures -- II Molecular Technologies -- 4 Nucleic Acid Blotting Techniques: Theory and Practice -- 5 DNA Amplification Techniques: An Overview -- 6 RT-PCR: Quantitative and Diagnostic PCR in the Analysis of Gene Expression -- 7 PCR-Based Methods for Mutation Detection -- 8 Nucleic Acid Hybridization and Amplification In Situ: Principles and Applications in Molecular Pathology -- 9 Gene Therapy -- III Applications to Molecular Pathology -- 10 An Overview of Molecular Genetics -- 11 Genetic Basis of Neurologic and Neuromuscular Diseases -- 12 Molecular Mechanisms of Endocrine Disorders -- 13 Molecular Pathogenesis of Cardiovascular Disease -- 14 Molecular Mutations in Human Neoplastic Disease: Application of Nucleic Acid Technology in the Assessment of Familial Risk, Diagnosis, and Prognosis of Human Cancers -- 15 Molecular Genetics and the Diagnosis of Hematological Malignancies -- 16 Molecular Techniques in Laboratory Diagnosis of Infectious Diseases -- IV Issues for the Clinical Molecular Pathology Laboratory -- 17 Quality Control Issues for the Clinical Molecular Pathologist -- 18 Prospects for the Future Role of the Clinical Laboratory

Medicine Pathology Medicine & Public Health Pathology


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