Title | Single-Cell Mutation Monitoring Systems [electronic resource] : Methodologies and Applications / edited by Aftab A. Ansari, Frederick J. de Serres |
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Imprint | Boston, MA : Springer US, 1984 |
Connect to | http://dx.doi.org/10.1007/978-1-4684-4664-7 |
Descript | XVIII, 290 p. online resource |
1. Somatic-Cell Mutation Monitoring System Based on Human Hemoglobin Mutants -- 1. Introduction -- 2. The Hemoglobin Mutants -- 3. Hemoglobin in Mutation Research: Gametal Mutation Rates -- 4. A System for Detecting Somatic Mutations of Hemoglobin -- 5. Methodological Aspects: Monospecific Anti-Mutant-Hemoglobin Antibodies -- 6. Methodological Aspects: Monoclonal Anti-Globin-Chain Antibodies -- References -- 2. Use of Fluorescence-Activated Cell Sorter for Screening Mutant Cells -- 1. Introduction -- 2. Immunologic Identification and Flow Detection of Erythrocytes Containing Amino Acid-Substituted Hemoglobin -- 3. Future of the Hemoglobin-Based Assay -- 4. Detection of Erythrocytes with Mutationally Altered Glycophorin A -- 5. Summary and Conclusions -- References -- 3. Development of a Plaque Assay for the Detection of Red Blood Cells Carrying Abnormal or Mutant Hemoglobins -- 1. Introduction -- 2. Principle of the Method -- 3. Reagents -- 4. Equipment -- 5. Procedure for the RBC-Antibody Plaque Assay -- 6. RBC-Protein A Plaque Assay -- 7. Conclusions -- References -- 4. Direct Assay by Autoradiography for 6-Thioguanine-Resistant Lymphocytes in Human Peripheral Blood -- 1. Introduction -- 2. Autoradiographic TGr T-PBL Assay Method -- 3. Sample Results -- 4. Statistical Analysis Methods -- 5. Discussion -- References -- 5. Application of Antibodies to 5-Bromodeoxyuridine for the Detection of Cells of Rare Genotype -- 1. Introduction -- 2. Materials and Methods -- 3. Results -- 4. Discussion -- 5. Summary -- References -- 6. Cytogenetic Abnormalities as an Indicator of Mutagenic Exposure -- 1. Introduction -- 2. Lymphocyte Assay Methodology -- 3. Chromosome Aberration Analysis Following Radiation or Chemical Exposure -- 4. The Analysis of Bone Marrow Samples -- 5. The Plausibility of Estimating Genetic or Carcinogenic Risk from Aberration Frequencies in Lymphocytes -- 6. Concluding Remarks -- References -- 7. Sister Chromatid Exchange Analysis in Lymphocytes -- 1. Introduction -- 2. Methodology -- 3. Selected Applications -- Appendix A. A Procedure for Growing and Preparing Human Lymphocytes for SCE Analysis -- Appendix B. A Procedure for Growing and Preparing Rat Lymphocytes for SCE Analysis -- References -- 8. Unscheduled DNA Synthesis as an Indication of Genotoxic Exposure -- 1. Introduction -- 2. Four Laboratory Approaches to Measuring Unscheduled DNA Synthesis -- 3. Methods -- 4. Reagents, Solutions, Stains, and Media -- 5. Equipment and Supplies -- References -- 9. The Micronucleus Test as an Indicator of Mutagenic Exposure -- 1. Historical Background -- 2. Rationale of the Test System -- 3. Technical Procedure -- 4. Results and Comparative Studies -- 5. Related Assay Systems -- 6. Advantages -- 7. Limitations -- 8. Application of the Micronucelus Test -- References -- 10. The Identification of Somatic Mutations in Immunoglobulin Expression and Structure -- 1. Introduction -- 2. Immunoglobulin Protein and Gene Structure -- 3. Methods for the Isolation of Mutants -- 4. Frequency and Phenotypes of Mutants -- 5. Discussion -- References -- 11. Detection of Chemically Induced Y-Chromosomal Nondisjunction in Human Spermatozoa -- 1. Introduction -- 2. Background -- 3. Agents That Increase YFF Bodies in Human Sperm -- 4. Discussion -- References