Title | Mammalian Cell Culture [electronic resource] : The Use of Serum-Free Hormone-Supplemented Media / edited by Jennie P. Mather |
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Imprint | Boston, MA : Springer US, 1984 |
Connect to | http://dx.doi.org/10.1007/978-1-4615-9361-4 |
Descript | XVIII, 284 p. online resource |
1 Serum Factor Requirements for the Initiation of Cellular Proliferation -- Multiple Serum Components Required for Cellular Proliferation -- EOF and Somatomedin C Can Replace the GQ/GI Progession Activity of PPP -- EGF and Somatomedin C Reduce the Minimum Gi Transit Time -- Availability of Free Somatomedin C Controls Minimum Gi Transit Time -- Somatomedin C Regulates Progression of Late Gi Phase and Commitment to DNA Synthesis -- EGF Is Required during Traverse of Early Gi -- Summary and Conclusions -- References -- 2 Regulation of Fibroblast Growth by Multiple Growth Factors in Serum-Free Medium -- In Vitro Models of Growth RegulationโWhy Do Cells Arrest Their Growth? -- Neoplastic Transformation and Relaxed Growth Regulation -- Replacing Serum with Defined Medium Components to Study Growth Regulation In Vitro -- Combinations of Growth Factors Are Required in Defined Media to Support Cell Proliferation -- Mechanism of Growth Factor ActionโEvents Stimulated by Growth Factors Prior to DNA Synthesis -- Comparison of the Growth Factor Requirements of Normal and Transformed Cells Grown in Serum-Free Media -- Conclusion -- References -- 3 Growth and Differentiation of Preadipocyte Cell Lines in Serum-Free Medium -- Results and Discussion -- References -- 4 Growth and Differentiation of Human Myelomonocyte Leukemia Cell Lines in Serum-Free Medium -- Human Myelomonocytic Cell Lines -- Growth of HL-60 in Defined Medium -- Differentiation of HL-60 in Defined Medium -- Involvement of Intracellular Cyclic Adenosine 3?:5?-Monophosphate (cAMP) in Differentiation of HL-60 -- Differentiation Effects of Agents That Increase Intracellular cAMP on HL-60 Primed with RA -- Growth and Differentiation of Other Myelomonocytic Cell Lines in Defined Medium -- References -- 5 In Vitro Immunization and Growth of Hybridomas in Serum-Free Medium -- In Vitro Immunization -- The Role of Serum -- Thiols -- Cellular Interactions and Soluble Factors -- Serum-Free Media -- Conclusion -- References -- 6 Kidney Cell Cultures in Hormonally Defined Serum-Free Medium -- Kidney Cell Culture in Serum-Supplemented Medium: MDCK and LLC-PKl as Model Systems -- Advantages of Kidney Cell Culture -- Limitations on Transport Studies with Kidney Cell Culture Imposed by Serum -- Limitations of Studies with Established Kidney Cell Lines: Importance of Primary Kidney Cell Culture -- Primary Kidney Culture and Hormonally Defined Medium -- Primary Culture of Specific Kidney Cell Types -- Use of Hormonally Defined Serum-Free Medium to Grow Specific Cell Types in the Kidney: The Concept of Selective Media -- Use of Primary Kidney Cell Culture to Study Medically Related Problems of the Kidney -- References -- 7 Rat Hepatocytes in Culture: A Model for Studies of Growth Control during Experimental Chemical Hepatocarcinogenesis -- Control of DNA Synthesis in Cultures of Adult Rat Hepatocytes -- Factors Required for the Induction of DNA Synthesis -- Platelet-Derived Growth Factor as an Inhibitor of Normal Hepatocyte DNA Synthesis -- Stimulatory Activity for Normal Hepatocyte DNA Synthesis in the Serum from Cancer Patients and Carcinogen-Fed Rats -- Altered Response of Hepatocytes from Carcinogen-Treated Rats to PDGF -- Discussion -- References -- 8 Intratesticular Regulation: Evidence for Autocrine and Paracrine Control of Testicular Function -- Testicular Cell Culture -- Autocrine Regulation of Testicular Cells -- Cell-Cell Interaction in the Testis -- Summary and Model of Intratesticular Regulation -- References -- 9 Attachment Factors in Cell Culture -- Collagen -- Fibronectin -- Laminin -- Chondronectin -- Serum-Spreading Factor -- Epibolin -- Fetuin -- Extracellular Matrix in Cell Culture -- Future Trends: Studies of Cell Shape and Cell-Substratum Interactions -- References -- 10 Cell Culture Studies Using Extracts of Extracellular Matrix to Study Growth and Differentiation in Mammalian Cells -- Background on Extracellular Matrix -- Cell Culture Methods Using Crude Extracts of Extracellular Matrix -- The Future -- References