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TitleHybridomas and Cellular Immortality [electronic resource] / edited by Baldwin H. Tom, James P. Allison
ImprintBoston, MA : Springer US, 1983
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Descript XX, 306 p. online resource


The ability to "immortalize" immunologically-useful cells by hybridization with a unique cancer cell has revolutionized serological studies and has revealed new potential applications in all fields of biological sciences. This volume presents the studies from a highly successful national symposium on Hybridomas and Cellular Immortality held November 1981 in Houston, Texas. The individual chapters exhibit the diversity of topics discussed during the meeting. These include emphasis on the origin of antibody diversity, Band T lymphocyte differentiation, applications of monoclonal antibodies in studies of histocompatibility, tumor, and viral antigens, plus the use of somatic cell hybridizations for studying T cell products. Three papers focus on the emerging methodologies of in vitro primary immunizations for both humoral and cell-mediated immunities, relevant for coupling with hybridoma technology. There is a useful mix of general (methods) and specific (applications) chapters. A unique aspect of the book is the presentation of both recent research findings with concise descriptions of the state of the art methodologies. It is anticipated that this work will be of interest to a wide audience of practioners in biomedical research. Hopefully, the information contained will foster new and imagiยญ native ideas in hybridoma applications. Baldwin H. Tom, Ph.D. James P. Allison, Ph.D. vii CONTENTS PART L INTRODUCTION TO HYBRIDOMAS 1 Somatic Cell Hybrids and Hybridomas Baldwin H. Tom 3 1. Somatic Cell Hybrids 8 Hybridomas. โ{128}ข โ{128}ข โ{128}ข โ{128}ข โ{128}ข 2


I. Introduction To Hybridomas -- 1 Somatic Cell Hybrids and Hybridomas -- II. Antibody Diversity And Lymphocyte Differentiation -- 2 The Nature and Origin of Antibody Diversity -- 3 Hematopoietic Tumors: Normal or Abnormal Models of Leukocyte Differentiation? -- 4 Dissection of Human Immunoregulatory T Lymphocytes: Implications for Understanding Clinical Disease -- 5 Immune (?) Interferon and Lymphotoxin Production by T Cell Lines and Hybrids -- III. Monoclonal Antibodies as Probes of Antigen Structure -- 6 Partial Structural Characterization of Human HLA-DR Subsets Using Monoclonal Antibodies -- 7 Monoclonal Antibodies for Analysis of HLA Antigens: Further Studies with the W6/32 Antibody -- IV. Monoclonal Antibodies in Diagnosis and Therapy -- 8 Monoclonal Antibodies to Hepatitis B Surface Antigen -- 9 Wandering Around the Cell Surface: Monoclonal Antibodies Against Human Neuroblastoma and Leukemia Cell Surface Antigens -- 10 Monoclonal Antibodies to Human Melanoma-Associated Antigen p97 -- 11 A Biochemical and Biosynthetic Analysis of Human Melanoma-Associated Antigens with Monoclonal Antibodies -- 12 Monoclonal Antibodies to a Tumor Specific Antigen on Rat Mammary Carcinoma Sp4 and their use in Drug Delivery Systems -- 13 Monoclonal Antibody to a Tumor Specific Epitope of Murine Lymphoma Cells: Use in Characterization of Antigen and in Immunotherapy -- V. New and Relevant Methodology -- 14 Human Hybridomas -- 15 Procedures for In Vitro Immunization and Monoclonal Antibody Production -- 16 Liposome-facilitated In Vitro Induction of Primary Cell-Mediated Immunity to Human Cancer Antigens: Potential Adjunct to Hybridoma Technology -- 17 Comparison of Three Immunoassays for Screening Anti-Hepatitis B Hybridomas -- VI. Poster Abstracts -- 1. T-Lymptocyte Subsets of Rhesus Macaque as Determined by Monoclonal Antibodies: T?, T?, T-NULL, and Histamine Receptor Bearing T Lymphocytes -- 2. Production of Human Immunoglobulin Secreting Clones Without the Use of H.A.T. Sensitive Fusion Partners -- 3. Species Distribution of Antigenic Sites on Mouse LDH-C4 Determined by Monoclonal Antibodies -- 4. A New Non-Immunoglobulin Producing Human Myeloma Cell line for Production of Hybridomas -- 5. Monoclonal Antibodies to Human Prostate and Bladder Tumor Associated Antigens -- 6. Antibody Purification by Recycling Isoelectric Focusing -- 7. Monoclonal Antibodies Against Human Ejaculated Sperm-Application in Antibody Mediated Infertility -- 8. Analysis of Peripheral Blood and Salivary Gland Lymphocytes in Sjogrenโ{128}{153}s Syndrome -- 9. A Monoclonal Antibody Which Identifies a Subpopulation of Human B Lymphocytes and Monocytes -- 10. In Vivo Localization of Tumor Associated Antigens with Radioactively Labeled Monoclonal Antibodies -- 11. Characteristics of Monoclonal Antibodies to Herpes Simplex Type 2 -- 12. Type Specific Monoclonal Antibodies to Equine Infectious Anemia Virus: Development and Use in the Characterization of Canine and Feline Derived Viruses -- 13. Biochemical and Biological Analysis of Venezuelan Equine Encephalomyelitis Viruses (VEE) Using Monoclonal Antibodies -- 14. Monoclonal Antibodies Specific for Herpes Simplex Virus Type 1 Mediate Antiviral Effects In Vitro and In Vivo -- 15. Monoclonal Antibodies Against Antigens of Amastigotes and Epimastigotes of Trypanosma Cruzi -- 16. Differential Effects of Four Monoclonal Antibodies to Human ?2-Microglobulin on In Vitro Lymphocyte Functions -- 17. Selective Inhibition of Functional Sites on Cell Bound Human C3b By Monoclonal Antibodies -- 18. Generation of Monoclonal Antibodies Reacting With Human Normal and Neoplastic Mammary Duct Epithelial Cells -- 19. Resolution of Human Liver Mao A and B Using A Monoclonal Antibody Immunoaffinity Column -- 20. Isolation of Antibodies that are Specific for Human Carboxypeptidase B But Not for the Zymogen Procarboxypeptidase B by Affinity Chromatography -- 21. Dexamethasone Effects on Hybridoma Formation -- 22. Class Antigen in the Rat: Evidence for More Than One Using Monoclonal Antibodies -- 23. An Immunohistochemical Method for Large Scale Specificity Screening of Monoclonal Antibodies -- 24. Monoclonal Antibodies to Chicken Hemopoietic-Lymphoid Cellular Differentiation Antigens -- Author Index

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