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TitleGene Transfer in the Cardiovascular System [electronic resource] : Experimental Approaches and Therapeutic Implications / edited by Keith L. March
ImprintBoston, MA : Springer US : Imprint: Springer, 1997
Connect tohttp://dx.doi.org/10.1007/978-1-4615-6277-1
Descript XX, 516 p. online resource

SUMMARY

The goal of gene transfer is protein expression. a process brought about by the insertion of a gene coding for a foreign protein into target cells resulting in the synthesis of the foreign protein For gene therapy, a tmnsferred therapeutic gene must be expressed at a level beneficial for the patient. This chapter provides an introductory overview of the rapidly evolving field of non-viral approaches for gene delivery to rnarnrnalian cells. Although currently there are fewer ongoing clinical trials using non-viral approaches than those using viral based systems, the number of non-viral trials is increasing. The long range goal of some research groups is the development of a genetically engineered artificial virus targeted to specific cells in the human body. An arurual conference, organized by Cambridge Healthtech Institute entitled "Artificial Self-Assembling Systems for Gene Transfer", brings together researchers interested in this field [1]. Assembly of an artificial virus is very complex; other research groups aim to develop simpler delivery systems consisting of a plasmid combined with delivery agents. Viral-based systems are very successful for gene delivery, but despite their successes, viral-based systems have some geneml limitations and system-specific limitations. When employing a viml-based system, the following limitations should be considered: โ{128}ข size limitation of the inserted gene due to packaging constraints (e. g. adenovirus, retrovirus) . โ{128}ข potential tumorigenesis (e. g. retrovirus) โ{128}ข potential for insertional mutagenesis (greater than plasmid based systems) โ{128}ข potential imrnunogenicity (e. g


CONTENT

Vectors and Gene Transfer Systems: Molecular Aspects of Delivery -- 1. Development of Viral Vectors for Human Gene Therapy: Retrovirus and Adenovirus (Part I) -- 2. Adenoviruses (Part II): Improvement of Adenoviral Vectors for Human Gene Therapy: E1 and E4 Deleted Recombinant Adenoviruses -- 3. Adeno-associated Virus and Other New DNA Virus Vectors -- 4. Plasmid and Other Non-Viral Vectors -- 5. The HVJ/Liposome Molecular Delivery System for In Vivo Genetic Engineering -- 6. Endogenous Expression Modification: Antisense Approaches -- Methods for Localizing Gene Transfer: Mechanical Aspects of Delivery -- 7. Catheter-Based Local Drug and Gene Delivery -- 8. Fluid Dynamics of Catheter Delivery: Effects on Delivery Efficiency and Localization -- 9. Targeted and Sustained-Release Delivery Concepts in Gene Therapy -- Gene Delivery for Local Vascular Expression -- 10. Viral Vector-Based Vascular Gene Delivery: Basic Studies and Therapeutic Applications -- 11. Cell-Based Vascular Gene Delivery: Endothelial Cells as Carriers -- 12. Cell-Based Gene Delivery: Smooth Muscle Cells as Carriers -- 13. Vascular Cell Proliferation Dynamics: Implications for Gene Transfer and Restenosis -- 14. Angiogenesis and Collateral formation -- Gene Delivery for Local Cardiac Expression -- 15. Cell-Based Myocardial Protein Delivery -- 16. Skeletal Myoblast Therapy in Cardiovascular Disease -- 17. Adenovirus and the Myocardium -- Gene Delivery for Systemic Expression -- 18. Gene Delivery for Systemic Expression: Plasmid, Retroviral, and Adenoviral Approaches -- 19. Adenoviral Gene Delivery Approaches for Systemic Expression -- 20. Experimental Approaches Using Kallikrein Gene Therapy for Hypertension -- Biophysical Considerations in Vector Delivery -- 21.Pharmacokinetics of Local Vector Delivery To Vascular Tissues: Implications for Efficiency and Localization -- Color Plates


Medicine Human genetics Cardiology Medicine & Public Health Cardiology Human Genetics



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