Title | Methods in DNA Amplification [electronic resource] / edited by Arndt Rolfs, Ines Weber-Rolfs, Ulrich Finckh |
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Imprint | Boston, MA : Springer US : Imprint: Springer, 1994 |
Connect to | http://dx.doi.org/10.1007/978-1-4615-2530-1 |
Descript | IX, 251 p. online resource |
General Aspects of PCR -- The Polymerase Chain Reaction and Fixed Tissues -- Impact of PCR on the Pathologistโs World -- Sensitive and Rapid Detection and Quantification of Nucleic Acids -- Polymorphic Keratins as Detected by PCR and SSCP -- Production of Antibodies of Monoclonal Specificity without the Use of Hybridoma Cell Lines -- Use of Chelex 100TM in the Extraction of Viruses from Diverse Cell-Free Clinical Samples for PCR -- Detection of Amplified Oncogenes by Differential Polymerase Chain Reaction -- Resolution of a Secondary Structure in an Unknown mRNA 5โEnd -- Automated Solid-Phase Sequencing of Genomic DNA Obtained from Polymerase Chain Reaction -- Alternative DNA-Amplification Methods -- Ligase-Mediated Detection Techniques -- Qualitative and Quantitative Detection of Nucleic Acids of Infectious Agents by NASBA -- Application of NASBA to the Detection of Listeria Monocytogenes -- PCR and Virological Problems -- Confirmation of Hepatitis C Virus Carrier State by Polymerase Chain Reaction in Cases with Nondiagnostic Serologies -- Confirmation of Hepatitis C Virus Positive Seras by Polymerase Chain Reaction -- Specific Detection and Rapid Identification of Human Enteroviruses by PCR Amplification -- Detection of Herpes Simplex Virus Using Polymerase Chain Reaction -- Detection of Human Cytomegalovirus (HCMV)-DNA from Granulocytes and Peripheral Blood Mononuclear Cells (PBMNC) of Immunosuppressed Patients by Nested PCR: Lack of Correlation to Isolation of Infectious Virus -- Timing of Mother-to Child Transmission of HIV-1 as Determinated by Nested Polymerase Chain Reaction - A Cohort Study in Kigali, Rwanda -- One-Day Detection of Enteroviruses in Clinical Specimens by Magnetic Bead Extraction of Viral RNA and Nested Polymerase Chain Reaction -- Monitoring of Poliovirus Strains Circulating in the Environment and Their Intertypic and Intratypic Differentiation by RFLP Analysis of a PCR Product Derived from the 5โNon-coding Region -- Detection of Adenoviruses in Blood by Polymerase Chain Reaction -- Detection of the HPV 16 E2 Gene in Nonkeratinizing Squamous Cervical Carcinomas by PCR -- PCR in the Field of Bacterial and Fungal Problems -- Identification of Toxoplasma-DNA by Polymerase Chain Reaction in Peripheral Blood Leukocytes of Patients with Suspected Toxoplasmosis -- Detection of Mycobacterium Tuberculosis in Clinical Samples by Polymerase Chain Reaction -- Tuberculosis in HIV Infected Patients Detected by PCR: A Comparison with Clinical Data -- Detection of Borrelia Burgdorferi in Human Skin Biopsies by a Nested Polymerase Chain Reaction -- The Application of PCR Fingerprinting to Epidemiological Analysis of Bacterial and Fungal Pathogens -- Contributors -- Suppliers of Special Items