Title | The Bile Acids: Chemistry, Physiology, and Metabolism [electronic resource] : Volume 4: Methods and Applications / edited by Kenneth D. R. Setchell, David Kritchevsky, Padmanabhan P. Nair |
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Imprint | Boston, MA : Springer US, 1988 |
Connect to | http://dx.doi.org/10.1007/978-1-4613-0901-7 |
Descript | 604 p. online resource |
1 Techniques for Extraction and Group Separation of Bile Acids -- 1. Introduction -- 2. Extraction -- 3. Purification and Group Separation -- 4. Hydrolysis and Solvolysis -- 5. Conclusions -- References -- 2 High-Performance Liquid Chromatography -- 1. Introduction -- 2. Chromatographic Behavior of Bile Acids -- 3. Detection Methods -- 4. Application of HPLC to Biological Specimens -- 5. Conclusions -- References -- 3 Nuclear Magnetic Resonance -- 1. Introduction: Why Use NMR for Bile Acids? -- 2. Basic Principles of NMR -- 3. Proton NMR of Bile Acids -- 4. 13C NMR of Bile Acids -- 5. Recent Advances in NMR Spectroscopy -- 6. Application of NMR to the Physical Chemistry of Bile Acids -- 7. NMR in the Biosynthesis and Metabolism of Bile Acids -- 8. ConclUding Remarks -- References -- 4 X-Ray Crystallography -- l. Introduction -- 2. X-Ray Diffraction by Single Crystals -- 3. Sample Preparation -- 4. Experimental Measurements -- 5. Structure Determination -- 6. Interpretation of Results -- 7. Pitfalls -- 8. Examples -- References -- 5 Mass Spectrometry of Bile Acids -- 1. Introduction -- 2. Principles and Instrumentation -- 3. Ionization Methods -- 4. Methods of Sample Introduction -- 5. Mass Spectrometric Fragmentation of Bile Acids -- 6. Quantitative Analysis of Bile Acids -- 7. Bile Acids Labeled with Stable Isotopes -- 8. Application of Mass Spectrometry to the Determination of Bile Acids in Biological Fluids -- 9. Compendium of Mass Spectra of Bile Acids -- References -- 6 Immunological Methods for Serum Bile Acid Analysis -- 1. Introduction -- 2. Physicochemical State of Bile Acids in Serum -- 3. Immunoassay -- 4. Preparation of Antigenic Bile Acids -- 5. Antibodies -- 6. Labeled Bile Acids -- 7. Assay Procedure -- 8. Comparison of Methods -- 9. Comparison between Methods -- 10. Enzyme Immunoassay -- 11. Serum Bile Acids in Physiology and Pathology -- References -- 7 Bioluminescence Assays Using Immobilized Enzymes in Bile Acid Analysis -- 1. Introduction -- 2. Bioluminescence Assays -- 3. Material -- 4. Methods -- 5. Results -- 6. Discussion -- 7. Summary -- References -- 8 Bile Acids in Extrahepatic Tissues -- 1. Introduction -- 2. Analytical Methods -- 3. Applications -- 4. Conclusions -- References -- 9 Tissue-Bound Bile Acids -- 1. Introduction -- 2. Methodology -- References -- 10 Serum Bile Acids -- 1. Introduction -- 2. Serum Bile Acids and the Different Enterohepatic Bile Acid Cycles in Normal Humans -- 3. Serum Bile Acid Measurements and the Dynamics of the Normal Enterohepatic Circulations -- 4. Serum Bile Acids and Liver Disease -- 5. Gas Chromatography-Mass Spectrometry and Monohydroxy Bile Acids in Liver Disease -- 6. Serum Bile Acid Composition in Other Circumstances -- 7. Conclusion -- References -- 11 Urinary Bile Acids -- 1. Introduction -- 2. Methodology -- 3. Bile Acids Detected in the Urine -- 4. Origin of the Atypical Bile Acids in the Urine -- 5. The Renal Clearance of Bile Acids -- 6. Conclusion -- References -- 12 Fecal Bile Acids -- 1. Introduction -- 2. Methods for Determination of Fecal Bile Acids -- 3. Fecal Bile Acid Excretion in Health -- 4. Fecal Bile Acid Excretion in Disease -- 5. Effect of Diet and Drugs on Fecal Bile Acid Excretion -- 6. Conclusions -- References