Title | The Polymerase Chain Reaction [electronic resource] / edited by Kary B. Mullis, Franรงois Ferrรฉ, Richard A. Gibbs |
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Imprint | Boston, MA : Birkhรคuser Boston : Imprint: Birkhรคuser, 1994 |
Connect to | http://dx.doi.org/10.1007/978-1-4612-0257-8 |
Descript | XXII, 458 p. online resource |
One: Methodology -- I. Basic Methodology -- 1. Manipulation of DNA by PCR -- 2. Cloning PCR Products -- 3. Optimization of Multiplex PCRs -- 4. Preparation of Nucleic Acids for Archival Material -- 5. PCR Amplification of Viral DNA and Viral Host Cell mRNAs in Situ -- II. Quantitation -- 6. Quantitative PCR: An Overview -- 7. Quantification of DNAs by the Polymerase Chain Reaction Using an Internal Control -- 8. RT-PCR and mRNA Quantitation -- 9. Analysis of Human T-Cell Repertoires by PCR -- III. Nonisotopic Detection -- 10. Ultrasensitive Nonradioactive Detection of PCR Reactions: An Overview -- 11. Fluorescent Detection Methods for PCR Analysis -- 12. Enzyme-Labeled Oligonucleotides -- 13. Application of the Hybridization Protection Assay (HPA) to PCR -- IV. Instrumentation -- 14. PCR Instrumentation: Where Do We Stand? -- 15. Rapid Cycle DNA Amplification -- 16. Automating the PCR Process -- V. Sequencing -- 17. PCR and DNA Sequencing -- 18. Phage Promoter-Based Methods for Sequencing and Screening for Mutations -- 19. Capture PCR: An Efficient Method for Walking Along Chromosomal DNA and cDNA -- Two: Applications -- I. General Applications -- 20. In Vitro Evolution of Functional Nucleic Acids: High-Affinity RNA Ligands of the HIV-1 rev Protein -- 21. The Application of PCR to Forensic Science -- 22. Recreating the Past by PCR -- 23. Nonbiological Applications -- II. Genetic Analysis -- 24. RT-PCR and Gene Expression -- 25. Fingerprinting Using Arbitrarily Primed PCR: Application to Genetic Mapping, Population Biology, Epidemiology, and Detection of Differentially Expressed RNAs -- 26. Genetics, Plants, and the Polymerase Chain Reaction -- III. Assessment of Therapy Effectiveness -- 27. PCR Assessment of the Efficacy of Therapy in Philadelphia Chromosome-Positive Leukemias -- 28. The Detection of Minimal Residual Disease (MRD) in Acute Lymphoblastic Leukemia Using Clone-Specific Probes Directed against V(D)J Junctional Sequences -- 29. Assessment of Therapy Effectiveness: Infectious Disease -- 30. Gene Therapy -- IV. Diagnostics -- 31. PCR and Cancer Diagnostics: Detection and Characterization of Single Point Mutations in Oncogenes and Antioncogenes -- 32. Clinical Applications of the Polymerase Chain Reaction -- 33. Infectious Diseases -- Three: PCR and the World of Business -- 34. PCR in the Marketplace -- 35. PCR and Scientific Invention: The Trial of DuPont vs. Cetus