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TitleThe Polymerase Chain Reaction [electronic resource] / edited by Kary B. Mullis, Franรงois Ferrรฉ, Richard A. Gibbs
ImprintBoston, MA : Birkhรคuser Boston : Imprint: Birkhรคuser, 1994
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Descript XXII, 458 p. online resource


James D. Watson When, in late March of 1953, Francis Crick and I came to write the first Nature paper describing the double helical structure of the DNA molecule, Francis had wanted to include a lengthy discussion of the genetic implications of a molecule whose strucยญ ture we had divined from a minimum of experimental data and on theoretical arguยญ ments based on physical principles. But I felt that this might be tempting fate, given that we had not yet seen the detailed evidence from King's College. Nevertheless, we reached a compromise and decided to include a sentence that pointed to the biological significance of the molecule's key feature-the complementary pairing of the bases. "It has not escaped our notice," Francis wrote, "that the specific pairing that we have postulated immediately suggests a possible copying mechanism for the genetic material." By May, when we were writing the second Nature paper, I was more confident that the proposed structure was at the very least substantially correct, so that this second paper contains a discussion of molecular self-duplication using templates or molds. We pointed out that, as a consequence of base pairing, a DNA molecule has two chains that are complementary to each other. Each chain could then act ". . . as a template for the formation on itself of a new companion chain, so that eventually we shall have two pairs of chains, where we only had one before" and, moreover, " ..


One: Methodology -- I. Basic Methodology -- 1. Manipulation of DNA by PCR -- 2. Cloning PCR Products -- 3. Optimization of Multiplex PCRs -- 4. Preparation of Nucleic Acids for Archival Material -- 5. PCR Amplification of Viral DNA and Viral Host Cell mRNAs in Situ -- II. Quantitation -- 6. Quantitative PCR: An Overview -- 7. Quantification of DNAs by the Polymerase Chain Reaction Using an Internal Control -- 8. RT-PCR and mRNA Quantitation -- 9. Analysis of Human T-Cell Repertoires by PCR -- III. Nonisotopic Detection -- 10. Ultrasensitive Nonradioactive Detection of PCR Reactions: An Overview -- 11. Fluorescent Detection Methods for PCR Analysis -- 12. Enzyme-Labeled Oligonucleotides -- 13. Application of the Hybridization Protection Assay (HPA) to PCR -- IV. Instrumentation -- 14. PCR Instrumentation: Where Do We Stand? -- 15. Rapid Cycle DNA Amplification -- 16. Automating the PCR Process -- V. Sequencing -- 17. PCR and DNA Sequencing -- 18. Phage Promoter-Based Methods for Sequencing and Screening for Mutations -- 19. Capture PCR: An Efficient Method for Walking Along Chromosomal DNA and cDNA -- Two: Applications -- I. General Applications -- 20. In Vitro Evolution of Functional Nucleic Acids: High-Affinity RNA Ligands of the HIV-1 rev Protein -- 21. The Application of PCR to Forensic Science -- 22. Recreating the Past by PCR -- 23. Nonbiological Applications -- II. Genetic Analysis -- 24. RT-PCR and Gene Expression -- 25. Fingerprinting Using Arbitrarily Primed PCR: Application to Genetic Mapping, Population Biology, Epidemiology, and Detection of Differentially Expressed RNAs -- 26. Genetics, Plants, and the Polymerase Chain Reaction -- III. Assessment of Therapy Effectiveness -- 27. PCR Assessment of the Efficacy of Therapy in Philadelphia Chromosome-Positive Leukemias -- 28. The Detection of Minimal Residual Disease (MRD) in Acute Lymphoblastic Leukemia Using Clone-Specific Probes Directed against V(D)J Junctional Sequences -- 29. Assessment of Therapy Effectiveness: Infectious Disease -- 30. Gene Therapy -- IV. Diagnostics -- 31. PCR and Cancer Diagnostics: Detection and Characterization of Single Point Mutations in Oncogenes and Antioncogenes -- 32. Clinical Applications of the Polymerase Chain Reaction -- 33. Infectious Diseases -- Three: PCR and the World of Business -- 34. PCR in the Marketplace -- 35. PCR and Scientific Invention: The Trial of DuPont vs. Cetus

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