Author | Rosenberg, Ian M. author |
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Title | Protein Analysis and Purification [electronic resource] : Benchtop Techniques / by Ian M. Rosenberg |
Imprint | Boston, MA : Birkhรคuser Boston : Imprint: Birkhรคuser, 1996 |
Connect to | http://dx.doi.org/10.1007/978-1-4757-1108-0 |
Descript | XXI, 434 p. online resource |
1 An Overview of this Manual -- 2 Protein Structure -- 3 Tracking the Target Protein -- 4 Electrophoretic Techniques -- 5 Getting Started with Protein Purification -- 6 Membrane and Particulate-Associated Proteins -- 7 Transfer and Detection of Proteins on Membrane Supports -- 8 Peptide Mapping and Microsequencing -- 9 Modified Proteins and Peptides -- 10 Chromatograph -- 11 Recombinant Protein Techniques -- A. Safety Considerations -- B. Antibody Preparation -- Production of Polyclonal Antisera in Rabbits -- Protocol B.1 Preparation of the Antigen-Adjuvant Emulsion -- Protocol B.2 Intramuscular Immunization (IM) -- Protocol B.3 Intradermal Immunization -- Protocol B.4 Subcutaneous Immunization -- Protocol B.5 Bleeding the Rabbit and Serum Preparation -- Protocol B.6 Precipitation of IgG with Saturated Ammonium Sulfate -- Purification of Antibody Using Protein A Affinity Columns -- Protocol B.7 Purifying Total Ig -- Numbering Mice -- C. Solutions -- Commercial Strengths of Common Laboratory Chemicals -- Water -- Molarity -- Choosing and Preparing Buffers -- Common Laboratory Solutions -- Extinction Coefficients -- D. Nucleic Acids -- Spectrophotometric Conversions -- DNA / Protein Conversions -- Oligonucleotide Concentrations -- Protocol D.1 DNA Quantitation Assay -- The Codon Dictionary -- Amino Acid Code Degeneracy -- Amino Acid and Codon Usage -- E. Modifications and Motifs -- Nomenclature -- Protein Modification Sequences -- Protein Kinase Recognition Motifs -- Subcellular Localization Motifs -- Protein Databases -- E Centrifugation -- Nomogram -- General Purpose Centrifuge Rotors. -- Ultracentrifuge Rotors -- G. Proteases and Proteolytic Enzyme Inhibitors -- Commonly Used Proteases -- Protocol G.1 Preparation of Defatted BSA -- Protease Inhibitors -- H. Radioactivity -- Manual and Machine Film Processing -- I. Miscellaneous -- Unit Prefixes -- The Greek Alphabet -- Abbreviations -- HPLC Pump Conversion -- Protocol I.1 Siliconizing Glassware -- J. List of Suppliers, Vendors, Manufacturers -- References