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TitlePlant and Insect Mycoplasma Techniques [electronic resource] / edited by M. J. Daniels, P. G. Markham
ImprintDordrecht : Springer Netherlands, 1982
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Descript 370 p. 66 illus. online resource


Only 14 years have passed since the first publication appeared which implicated mycoplasmas as agents of plant disease. The diseases themยญ selves have been known for much longer; indeed clover phyllody, a typical example, was described in the seventeenth century, well before any animal mycoplasma diseases had been documented. The early history of plant mycoplasmas is described in Chapter 2 and one obvious conclusion to be drawn from the frustrating experiences of the earlier workers is that the experimental methods at their disposal were simply inadequate for the task. Progress in science depends critically upon the development of new methods. Although important advances have been made in plant and insect mycoplasmology, notably in the discovery of spiroplasmas, many intractable problems remain. Most plant mycoยญ plasmas cannot yet be cultured in vitro, and their natural plant habitat, the phloem, is one of the most difficult plant tissues for the experiยญ menter to handle, placing severe restrictions on the type of experiments which can be performed in vivo. It is clear that radically new methods may be required to solve these problems. A survey of the progress which has been made shows that application of techniques from a wide range of disciplines has been necessary. A successful individual or group of workers must possess the skills of a plant pathologist, a plantsman, a plant physiologist, a light-and electron microscopist, a bacteriologist, a biochemist, an immunologist, an entoยญ mologist, a virologist and a molecular geneticist


1. The Biology of Mycoplasmas -- 2. Plant and Arthropod Mycoplasmas: A Historical Perspective -- 3. The โ{128}{152}Yellowsโ{128}{153} Plant Diseases: Plant Hosts and their Interaction with the Pathogens -- 4. Light and Electron Microscopy -- 5. Chemical Treatment for Control of Plant Mycoplasma Diseases -- 6. Media and Methods for Culture of Spiroplasmas -- 7. Culture of Other Microorganisms from Yellows- diseased Plants -- 8. Serological Characterisation of Spiroplasmas and Other Mycoplasmas -- 9. Physicochemical Characterisation of Plant and Insect Mycoplasmas -- 10. Insect Vectors -- 11. Miscellaneous Methods -- Notes on Contributors

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