This research aimed to 1) develop the structure of life values of student teachers in Rajabhat Universities 2) determine the life values of student teachers in Rajabhat Universities and 3) analyze the factors of value in terms of student level and university level that affecting life value of student teachers in Rajabhat Universities. The main tool was developing from the structure of life values of student teachers in Rajabhat Universities which was referent to Rokeach idea of Instrumental Values in 1973. The samples of this study were collected from 1,324 student teachers, 442 teachers, 147 officers, and 145 people in community in 30 Rajabhat Universities in Thailand. The instruments were separated into 4 versions which classified by different sources as student version, teacher version, officer version, and community version with its reliability between 0.65-0.96. Develop the structure of life values of student teachers in Rajabhat Universities by the factor analysis and second order confirmatory factor analysis of the linear structural equation relationship: LISREL. The final step was applied by using the hierarchical linear model analysis by HLM program. The major results were as follows: 1. The structure of life values of student teachers in Rajabhat Universities included 13 factors; Ambition, Capable, Lively, Empathy, Honest, Imagination, Intellectual, Logical, Self- Controlled, Love, Polite, Responsible and Love Nature 2. Student teachers in Rajabhat Universities were in the high-level of life values in every factor. However, Lively was in the top rank of high-level and Ambition was in the low rank of level. 3. Student level factors that influence positively on life values of Student teachers in Rajabhat Universities were the expectation of a masterFs degree, Democratic-Rearing and attitude toward teaching career. The factors that influence negatively on life values were other majors than mathematics, science, languages and humanity included the GPA that below 2.51 was effect. University level factor that influence positively on life values was an atmosphere of the university.