ประสิทธิภาพของวิธีการตรวจเซลล์เยื่อบุช่องคลอดและการเกิดผลึกของเมือกที่เก็บจากช่องคลอดในการตรวจการเป็นสัดของสุกรนาง / ณ พัทธ์ ปัณฑุกำพล = Efficiencies of vaginal cytology and arborization of vaginal mucus for oestrous detection in sow / Naphat Panthukumphol
This study aim to determine the efficiency of vaginal cytology and vulvar arborisation for individual oestrous detection in sow and to compare the efficiency of two difference protocol were used in vaginal cytology. Moreover, this study was also focus on the effect of two difference managements. The study was divided into two parts. Part I performed in 20 weaning cross-breed (LWxLR) sows within 2nd-4th parity in the farm. Part II performed in 5 weaning cross-breed (LWxLR) sows in organic pig project in Nan province. The samples were collected once a day since the weaning day until the sows were inseminated for a couple of day. Sample collection compose of the arborisation (impression smear at the vulvar) and the vaginal cytology (flushing with sterile normal saline and swabbing with moistened cotton swab). Inspection of behavioral change and ultrasound technique were also performed. Part I: 95% (19/20) of sows showed the arborisation. 20% (4/20) of sows showed exactly on the ovulation day. 60 (12/20) and 50% (10/20) of sows showed before and after the ovulation day, respectively. Score +2 was found the most. This score was likely to be found on d-1 and d+1 at 73.68% (14/19). The flushing vaginal cytology was found that 61.87% (86/139) of samples can be found epithelium cells. The proportion of each cell type (small intermediate, large intermediate, superficial) were found on d-3 (52.70±23.96, 25.00±14.14, 19.30±19.25), d-2 (71.47±19.79, 15.33±10.43, 11.20±15.36), d-1 (59.62±24.96, 20.38±14.64, 20.00±23.00), d0 (59.00±37.48, 18.08±13.93, 18.31±22.22), d+1 (38.85±31.10, 40.38±22.77, 20.77±13.67), and d+2 (58.75±14.36, 32.50±18.93, 8.75±8.54). The swabbing vaginal cytology was found that 91.37% (127/139) of samples can be found epithelium cells. The proportion of each cell type were found on d-3 (62.65±21.15, 24.75±12.72, 12.60±15.27), d-2 (60.15±22.52, 23.00±14.27, 17.35±20.85), d-1 (41.32±19.71, 33.68±15.44, 25.00±21.86), d0 (51.25±28.28, 26.00±16.67, 22.75±23.76), d+1 (20.29±15.46, 37.35±14.37, 42.35±24.88), and d+2 (22.00±11.51, 38.00±10.37, 40.00±18.71). Part II: 75% (3/4) of sows were show the arborisation. All of shown arborisation occurred on the ovulation day with score +2. The flushing vaginal cytology was found that 86.20% (25/29) of samples can be found epithelium cells. The proportion of each cell type were found on d-3 (41.67±37.53, 18.33±18.93, 40.00±43.59), d-2 (76.67±15.28, 10.00±10.00, 16.67±20.82), d-1 (30.00±14.14, 26.25±17.97, 43.75±20.56), d0 (45.00±37.75, 18.00±9.08, 37.00±34.57), d+1 (31.25±23.94, 33.75±14.93, 35.00±33.17), and d+2 (17.50±10.61, 52.50±24.75, 30.00±14.14). The swabbing vaginal cytology was found that 86.67% (26/30) can be found epithelium cells. The proportion of each cell type were found on d-3 (26.67±25.17, 23.33±5.77, 50.00±26.46), d-2 (15.00±21.21, 35.00±21.21, 50.00±42.43), d-1 (31.25±36.14, 23.75±4.79, 50.00±35.59), d0 (46.00±40.53, 26.00±16.36, 32.00±25.88), d+1 (31.67±23.63, 35.00±18.03, 46.67±35.12), and d+2 (37.50±31.82, 22.50±3.54, 22.50±3.54). In conclusion, The vulvar arborisation tends to have some relationship with the ovulation time, especially in organic pig in Nan province. The vaginal cytology is also relate with ovulation time, but the specificity is still low, especially in flushed sample. In practice, we suggest to detect the decreasing of small intermediate cell instead of the rising of superficial cell. Furthermore, the protocol should be developed in the future to get higher reliable result.
