Introduction: The wear resistance of artificial denture teeth is a very importance physical property in determining the function and service life of the removable dental prosthesis. The ability of artificial denture teeth to maintain a stable occlusal relationship over time relies upon this property, but wear testing on purely outer enamel surface is difficult and questionable. Therefore, the purpose of this study was to evaluate the wear resistance of artificial denture teeth in the sub-enamel layer. Material and methods: Four types of artificial denture teeth consisted of 3 conventional acrylic resin teeth (Cosmo HXL, Major Dent and Yamahachi FX), 1 high cross-linked acrylic resin teeth (Trubyte Bioform IPN), 2 composite resin teeth (SR Orthosit PE and Yamahachi PX), and 1 porcelain teeth (ACE Teeth). The slap sub-enamel surface of each type was used to evaluate. The 2-body wear test was performed in custom made pin on disc apparatus (15 N, 1000 rpm, 10,000 cycles) with aluminum oxide antagonist. Volume loss and weight loss were measured. Data were statistically analyzed with 1-way ANOVA, followed by Tamhane’s multiple range post hoc tests (α=.05). The generalized linear model between volume loss and weight loss was also analyzed. Results: The wear of each artificial denture teeth: Cosmo HXL, Major Dent, Yamahachi FX, Trubyte Bioform IPN, SR Orthosit PE, Yamahachi PX; and ACE Teeth in volume loss were 0.210±0.078 0.071±0.043 0.054±0.016 0.693±0.197 0.040±0.009 0.056±0.019 and 0.054±0.008 mm3, respectively, in weight loss were 0.8±0.3 0.2±0.1 0.2±0.1 1.4±0.2 0.3±0.1 0.3±0.1 and 0.2±0.2 mg, respectively. Wear from Trubyte Bioform IPN was significantly higher than that of others (p<0.001). ACE Teeth, SR Orthosit PE and Yamahachi PX were not totally demonstrated the higher wear resistance than 3 conventional acrylic resin teeth tested. There were a relationship between volume loss and weight loss. Conclusion: Wear resistance varied among the denture tooth materials. Wear resistance of Trubyte Bioform IPN was lower than the others.