In this work, nanoparticles of calcium alginate-chitosan and calcium alginate-positively charged chitosan derivatives used for entrapment of glucosamine were prepared. The particle formation was based on ionotropic gelation of cationic polymers (i.e. chitosan, N,N,N-trimethylammonium chitosan chloride (TMC), N-[(2-hydroxyl-3-trimethylammonium) propyl]chitosan chloride (HTCC) and an anionic polymer. The parameters affecting the formation, method of entrapment of glucosamine, entrapment efficiency, stability and release profile of glucosamine form nanoparticles were investigated. The results indicated that sonication and concentration of cationic polymers affected the size of nanoparticles. The optimal sonication time was 20 min and 0.15 mg/ml of cationic polymers should be used in the process. To get highest entrapment efficiency, glucosamine should be added in alginate solution. The in vitro release of glucosamine-loaded nanoparticles was investigated in phosphate buffer solution at pH 7.4. The results showed that glucosamine was released at an almost constant rate in the first 6 h. After that the release rate decreased and halted until the test period of 24 h. In addition, the stability of glucosamine-loaded nanoparticles in aqueous solution and in dry forms was studied at room temperature. The results indicated that the nanoparticles in aqueous solution for as long as 60 days tended to aggregate and precipitate while the size of dried glucosamine-loaded nanoparticles was slightly changed.
SUBJECT
อนุภาคนาโน
วัสดุโครงสร้างนาโน
กลูโคซามีน
ข้อเสื่อม
ข้อเสื่อม -- การรักษา
ยา -- การควบคุมการปลดปล่อย
Nanoparticles
Nanostructured materials
Glucosamine
Osteoarthritis
Osteoarthritis -- Treatment
Drugs -- Controlled release
LOCATION
CALL#
STATUS
Thailand and ASEAN Information Center (6th Floor) : Chula Collection
