บทบาทของไมโทเจน–แอคทิเวเตดโปรตีนไคเนสต่อการแสดงออกของเด็นทีนไซเอโลฟอสโฟโปรตีนในเซลล์เนื้อเยื่อในฟันของมนุษย์เมื่อกระตุ้นด้วยอะซีแมนแนน / วิสากานต บุญไพศาลเสรี = The role of mitogen activated protein kinase in dentine sialophosphoprotein expression in human dental pulp cell stimulated with acemannan / Wisakarn Boonpaisanseree
In this study, effect of acemannan on dentine sialophosphoprotein expression in human dental pulp cell via mitogen activated protein kinase (MAPK) signal pathway were investigated. Primary human dental pulp fibroblasts were treated with acemannan. The expression of DSPP mRNA was determined by Reverse Transcription- Polymerase Chain Reaction (RT-PCR) technique. The phosphorylation of MAPK, with and without specific MAPK inhibitor namely U0126(ERK inhibitor), SP600125(JNK inhibitor) and SB203580 (p38MAPK inhibitor), were determined by western blot analysis. Acemannan, at 4 mg/ml, significantly stimulated DSPP mRNA expression. Treated with acemannan, the phosphorylation of ERK1/2, JNK and p38 MAPK were reached maximum level at 45, 15 and 15 minutes of incubation, respectively. At these times of incubation, acemannan stimulated phosphorylation of ERK1/2, JNK and p38 MAPK up to 1.64±0.6, 2.75±0.16 and 3.11±0.09 fold compared with the untreated group, respectively. Pretreated with specific inhibitors of MAPK (U0126, SP600125 and SB203580) resulted in 86, 46 and 51 percentage reduction of the phosphorylation levels of ERK1/2, JNK and p38 MAPK, respectively, compared with these of acemannan-treated group. In conclusion, with this study, acemannan was able to enhance the DSPP mRNA expression in human dental pulp cells and involved with MAPK signaling pathway.
