บทบาทของซีเมนต์อุดคลองรากฟันต่อแบคทีเรียในระบบคลองรากฟัน / พรทิพย์ แซ่อึ้ง = Role of root canal cement on bacteria in root canal system / Porntip Sae-ung
Root canal filling materials are believed to play roles in managing endodontic bacteria by entombing them in inaccessible area such as dentinal tubules. Previous studies showed that flowability of AH plus were superior to Zinc oxide eugenol (ZOE) cement. The objective of this study was to determine the ability of root canal cements with different flowability to inhibit bacterial regrowth from dentinal tubules. Dentine cylinders were infected with Enterococcus faecalis. The samples were then instrumented upto size 50 file and the smear layer was removed. The samples were assigned into groups according to root filling materials: Gutta percha (GP) + AH plus, GP+ ZOE, GP without cement (Positive control) and Flowable composite (Negative control). GP and cement on the root canal walls were removed and BHI broth was placed in the root canal. Bacteria regrowth in the root canal was investigated for up to 30 days in culture. Results showed that bacteria in the dentinal tubules had potential to repopulate the root canal after obturation. The AH plus group had fewer samples with bacterial regrowth than the ZOE group at 30% and 70% respectively. The length of time before bacterial regrowth was also longer in AH plus than in ZOE group. Neither AH plus nor ZOE cements can completely entomb bacteria in the dentinal tubules and prevent them from repopulating the root canal.
