การปรับสภาพเบื้องต้นของเศษรากมันสำปะหลังในน้ำร้อนอัดความดัน / ธนกร นวรัตนไพบูลย์ = Pretreatment of cassava root waste in hot compressed water / Tanakorn Navaruttanapaibool
The hydrolysis in hot compressed water (HCW), or liquid hot water (LHW), was reported as a promising pretreatment of lignocellulosic materials for ethanol production. In this work, the cassava root waste was used as the lignocellulose sample. After the pretreatment process, liquid obtained from batch reactor was tested for quantifying pentose and hexose using high-performance liquid chromatography, whilst the solid sample was fed to enzymatic digesting process for measure a pretreatment efficiency. The variables that can affect the pretreatment efficiency were studied, including temperature, pressure, solid: water ratio and pretreatment time. For enzymatic digesting process, cellulase was applied to the pretreated cassava root in order to hydrolyze accessible cellulose to glucose. Note that the process variables for enzymatic digesting were fixed at pH 4.8 of citrate buffer for 48 hrs. The optimal pretreatment condition was located from the glucose concentration founded in liquid enzymatic hydrolysate (LEH). At the optimum pretreatment conditions, the highest glucose concentration of approximately 32 g/L in LEH was observed.
