ผลการต้านเชื้อพอร์ไฟโรโมแนส จิงจิวาลิส และ เชื้อพรีโวเทลลา อินเตอร์มีเดียของน้ำยาบ้วนปากผสมสารสกัดโพลีแซคคาไรด์จากเปลือกทุเรียน / รัชนู รูปทรง = The antimicrobial effect against Porphyromonas gingivalis and Prevotella intermedia of mouthrinse containing the extracted polysaccharide from durian fruit-halls / Rutchanoo Roobsong
Objective To investigate the antimicrobial effect against Porphyromonas gingivalis and Prevotella intermedia of 2% w/w concentration mouthrinse containing the extracted polysaccharide from durian fruit hulls in vitro. Materials and methods This study investigated the antimicrobial effect against Porphyromonas gingivalis ATCC 33277and Prevotella intermedia ATCC 25611 by Disc diffusion technique and Time kill analysis, recorded at 30 seconds,1 and 3 minutes by counting the colony forming unit which compare with 0.1% chlorhexidine mouthrinse and 0.9% normal saline solution. Results This study found that mouthrinse containing the extracted polysaccharide from durian fruit hulls had statistically significant antimicrobial effect against Porphyromonas gingivalis and Prevotella intermedia (p<0.001) when compare with normal saline solution. The inhibition zones indicating the two bacteria were inhibited from the mouthrinse were 11.0 and 15.5 millimeters, respectively. In time kill analysis, Porphyromonas gingivalis and Prevotella intermedia were killed at 100 % when the bacteria contacted with mouthrinse for 30 seconds, 1 and 3 minutes. The inhibition zones of mouthrinse containing the extracted polysaccharide from durian fruit hulls were less than those of chlorhexidine mouthrinse significantly (p<0.001). However, the bactericidal effect of mouthrinse containing the extracted polysaccharide from durian fruit hull was not different when compare with chlorhexidine mouthrinse. Conclusion: 2% w/w concentration mouth-rinse containing the extracted polysaccharide from durian fruit hull has an antimicrobial effect against Porphyromonas gingivalis and Prevotella intermedia 100 % at 30 seconds.
SUBJECT
พอร์ไฟโรโมแนส จิงจิวาลิส
สารต้านแบคทีเรีย
น้ำยาบ้วนปาก
ทุเรียน
พอลิแซ็กคาไรด์
Porphyromonas gingivalis
Antibacterial agents
Mouthwashes
Durian
Polysaccharides
Porphyromonas gingivalis -- Drug effects -- Growth & development
Prevotella intermedia -- Drug effects -- Growth & development