Cellulose is cracked by hydrothermal catalytic process to give glucose and derivatives glucose. It can be one of the key technologies for a future sustainable society using cellulose biomass. This study was to investigate the factors that could make the effects to catalytic hydrolysis reaction of cellulose. The factors were included with temperature (150 - 250 ℃), reaction time (5 - 30 minutes), pressure (5 - 7 MPa) and cellulose/catalyst ratio (1:0.25 - 1:0.75). The reaction was carried out in a stainless steel autoclave with capability of 250 ml by water media and used Avicel (Merck, microcrystalline) as cellulose. 20 wt% NiO/ZSM-5 prepared by co-impregnation technique was used as catalyst in this study. Liquid-phase products were analyzed by HPLC. Residue cellulose and catalyst were filtrated and weighted for calculation. Cellulose conversions were determined by the change in the weight of cellulose loaded before and after the reaction, and selectivity are reported on a carbon basis. From the results, The optimal condition for catalytic hydrolysis reaction of cellulose to synthesis glucose and glucose derivatives was in temperature of 230 ℃, reaction time of 5 minutes, pressure of 6 MPa and cellulose/catalyst ratio of 1:0.5.