I: Principles of laboratory medicine -- Introduction to laboratory medicine -- Selection and analytical evaluation of methods-with statistical techniques -- Clinical evaluation of methods -- Evidence based laboratory medicine -- Establishment and use of reference values -- Preanalytical variables and biological variation -- Specimen collection and processing -- Quality management -- II: Analytical techniques and instrumentation -- Principles of basic techniques and laboratory safety -- Optical techniques -- Electrochemistry and chemical sensors -- Electrophoresis -- Chromatography and extraction -- Mass spectrometry -- Enzyme and rate analyses -- Principles of immunochemical techniques -- Nucleic acid techniques -- Microfabrication and microfluidics and their application to clinical diagnostics -- Automation in the clinical laboratory -- Point-of-care testing -- III: Analytes -- Amino acids, peptides, and proteins -- Serum enzymes -- Enzymes of the red blood cell -- Tumor markers -- Kidney function tests -- Carbohydrates -- Lipids, lipoproteins, apolipoproteins, and other cardiovascular risk factors -- Electrolytes and blood gases -- Hormones -- Catecholamines and serotonin -- Vitamins and trace elements -- Hemoglobin, iron, and bilirubin -- The porphyrias and other disorders of porphyrin metabolism -- Therapeutic drugs and their management -- Clinical toxicology -- Toxic metals -- IV: Molecular diagnostics and genetics -- Principles of molecular biology -- Genomes and nucleic acid alterations -- Nucleic acid isolation -- Inherited diseases -- Identity assessment -- Molecular methods in diagnosis and monitoring of infectious diseases -- Pharmacogenetics -- Hematopoietic malignancies -- Plasma nucleic acids -- V: Pathophysiology -- Diabetes mellitus -- Cardiovascular disease -- Kidney disease -- Physiology and disorders of water, electrolyte, and acid-base metabolism -- Liver disease -- Gastrointestinal and pancreatic diseases -- Disorders of bone and mineral metabolism -- Pituitary function and pathophysiology -- The adrenal cortex -- The thyroid: pathophysiology and thyroid function testing -- Reproductive endocrinology and related disorders -- Pregnancy and its disorders -- Newborn screening and inborn errors of metabolism -- Hemostasis -- VI: Appendix -- Reference information for the clinical laboratory

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