To present fabrication of direct methanol fuel cell (DMFC). Stainless steel plate and stainless steel mesh were used as electrode. While Nafion and Neosepta membrane were used as electrolyte membrane. Optimization of the fuel cell and investigation of its equivalent circuit was performed. It was found that the best fuel cell was obtained from the following condition. The DMFC should have 1 cm2 active area, having stainless steel plate as electrode material. The anode and cathode were modified with 0.9 mg/cm² Pt-Ru and 0.3 mg/cm² Pt, respectively. Nafion 117 membrane should be activated before using. The MEA should be hot pressed at temperature of 150ºC with pressure of 30 kg/cm². It was found that open circuit voltage, maximum current, maximum power and fuel cell impedance were 0.522 V, 12.2 mA, 1.14 mW and 10 Ω respectively, when the DMFC was supplied with 2 M methanol under static condition to the anode and 1 l/min at 50 kPa of oxygen to cathode.