A 12 – week feeding trial was carried out to determine the effect of different supplementation of vitamin C on growth, survival and low salinity tolerance of the settled juvenile spotted babylon, Babylonia areolata, under flow-through seawater system. Juveniles (0.37 ± 0.01 cm) were fed to apparent satiation on two types of foods (natural food or artificial diet), varying in 5 levels of vitamin C (ascorbyl-2-polyphosphate; APP) supplementations (0, 100, 200, 500 and 1000 mg / 1 kg diet). Results showed that growth in term of shell length and weight of juveniles fed on artificial diet supplemented with 200 mg / 1 kg diet was significantly higher than those fed on other diet supplemented vitamin C diets (P<0.05) and growth of juvenile fed on the artificial diet was significantly higher than those fed the natural diet in all vitamin C supplementations. No significant difference in survival rate was found among all feeding trials (P<0.05). At the end of the experiment, a low salinity stress test (20 ppt) indicated that survival of juveniles fed artificial diet supplemented with any vitamin C levels was not significantly difference. However, survival of juvenile fed on the artificial diet with 100 mg vitamin C / kg was significantly higher than those juveniles fed the natural diet with all vitamin C supplementations.