Evolutionary engineering of industrially important microbial phenotypes / Stephen S. Fong -- Improving protein functions by directed evolution / Nikhil U. Nair and Huimin Zhao -- Engineering DNA and RNA regulatory regions through random mutagenesis and screening / Ichiro Matsumura, Sean A. Lynch, and Justin P. Gallivan -- Evolving pathways and genomes for the production of natural and novel compounds / Ethan T. Johnson and Claudia Schmidt-Dannert -- Models predicting optimized strategies for protein evolution / Jonathan J. Silberg and Peter Q. Nguyen -- Low-copy number plasmids as artificial chromosomes / Kristala L. Jones Prather -- Chromosomal engineering strategies / Kenan C. Murphy -- Regulating gene expression through engineered RNA technologies / Maung Nyan Win and Christina D. Smolke -- Tools designed to regulate translational efficiency / Claes Gustafsson -- Metabolic engineering of the secretory processing pathway in eukaryotes / Mohak Mhatre, Maira P. Pellegrini, and Michael J. Betenbaugh -- Engineering multifunctional enzyme systems for optimized metabolite transfer between sequential conversion steps / Robert J. Conrado, Thomas J. Mansell, and Matthew P. DeLisa --