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TitleMethods in alcohol-related neuroscience research [electronic resource] / edited by Yuan Liu, David M. Lovinger
Imprint Boca Raton : CRC Press, [2002]
Connect tohttp://marc.crcnetbase.com/isbn/9781420042092
Descript 391 p., [4] p. of plates : ill. (some col.)

CONTENT

Machine generated contents note: Chapter 1 Quantitative Trait Locus (QTL) Mapping in Mice1 -- Abraham A. Palmer and Tamara J. Phillips -- Chapter 2 Knockout and Knockin Mice 31 -- Gregg E. Homanics -- Appendix 2.1 Preparation of Mouse Embryonic Fibroblast Feeder Cells 57 -- Appendix 2.2 ES Cell Transformation by Electroporation 59 -- Chapter 3 Molecular Studies by Chimeragenesis and Mutagenesis 63 -- Michael J. Beckstead, Gregory E Lopreato, Kent E. Vrana and S. John Mihic -- Chapter 4 Combining Patch-Clamp Recording and Gene Profiling -- in Single N eurons83 -- Hermes H. Yeh, Stavros Therianos and Shao-Ming Lu -- Appendix 4.1 Protocol for Single-Cell mRNA Amplification/Reverse -- N orthern A nalysis99 -- Appendix 4.2 Protocol for Single-Cell RT-PCR 109 -- Appendix 4.3 Protocol for Single-Cell Multiplex RT-PCR 111 -- Chapter 5 Quantitative Analysis of Neurotransmitter Receptor -- Expression with Antibody-Based Techniques 113 -- C. Fernando Valenzuela and Daniel D. Savage -- Appendix 5.1 Detailed Protocols for Western Immunoblotting139 -- Chapter 6 Artificial Bilayer Techniques in Ion Channel Study143 -- Steven N. Treistman, Robert O'Connell and John Crowley -- Chapter 7 Rapid Drug Superfusion and Kinetic Analysis159 -- David M. Lovinger, Suzanne Sikes and Qing Zhou -- Chapter 8 Electrophysiological Assessment of Synaptic -- Transmission in Brain Slices 191 -- Jeff L. Weiner -- Chapter 9 Cognitive Correlates of Single Neuron Activity -- in Task-Performing Animals 219 -- Bennet Givens and T Michael Gill -- Chapter 10 Multichannel Neural Ensemble Recording During -- Alcohol Self-Administration241 -- Patricia H. Janak -- Chapter 11 Combined Single-Neuron Recording and Microdialysis -- in the Brain of Freely Behaving Animals (Rodents -- and Non-Human Primates) 259 -- Nandor Ludvig -- Chapter 12 Quantitative Microdialysis for in vivo Studies -- of Pharmacodynamics 287 -- Rueben A. Gonzales, Amanda Tang and Donita L. Robinson -- Chapter 13 Structural Magnetic Resonance Imaging (MRI) -- of the Human Brain 319 -- Terry L. Jerigan -- Chapter 14 Biochemical, Functional and Microstructural -- Magnetic Resonance Imaging (MRI) 345 -- Elfar Adalsteinsson, Edith V. Sullivan and Adolf Pfefferbaum -- Index 373


Alcohol -- Physiological effect -- Research -- Methodology Brain -- Pathophysiology -- Research -- Methodology Alcoholism -- Molecular aspects -- Research -- Methodology Electronic books.



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