Atrazine [2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine] is one of the most widely used herbicides in the world for the control of annual grasses and broadleaf weeds in corn and sorghum. It is also used as a nonselctive herbicide for vegetation control in non crop land. This research investigates the fungi capable of the biodegradation of atrazine into the less toxic metabolite. Twenty-eight isolates of the endophytic fungi, soil fungi and white-rot fungi were screened for their degradation ability in synthetic medium using spectrophotometry analysis. The fungus isolate W5, Trametes versicolor had the highest degradation rate of atrazine (0.75 milligrams per grams dry weight) after 20 days. The secondary degradation test was carried out with a Height Performance Liquid Chromatography (HPLC) analysis as a quantitative measurement of atrazine degradation for 42 days of incubation. The results showed that atrazine concentration decreasd gradually and atrazine concentration was 2.66 milligrams per liter on day 42. The result of degradation test related to the growth of the fungus. The optimum growing and degrading condition for the fungus isolate W5 were achieved using atrazine concentration 10 milligrams per liter of and glucose concentration 20 milligrams per liter of at pH 5 at 98.94 percentage of degradation efficiency. The metabolite of degraded atrazine detected by Liquid Chromatography Mass Spectroscopy (LC-MS) on day 35 was 2-hydrox-4-(isopropylamino)-6-(ethylamino)-striazine (OIET)
