This research was divided in two parts. The first part was the extraction of dyestuff of jackfruit stems. The analysis with 2[superscript k] factorial was applied to determine the effect of parameters, which were temperature, time and ratio of dry wood to water. The results showed that the ratio of dried wood to water was the major effect to the dyestuff extraction following by time and temperature. From the 2[superscript k] factorial results could induce to find the optimum extraction condition, which were 90 ํC, 150 minutes and 1: 40 ratio of dry wood to water. The second part was the study of dyeing condition of silk and cotton fabrics with the extracted dyestuff from jackfruit stems. The optimum dyeing condition of silk and cotton fabrics without using mordant was 100 ํC, 40 minutes, liquor ratio 50 : 1 with its solution condition and 80 ํC, 40 minutes, liquor ratio 30 : 1 at pH 7 respectively. The using mordant alum iron and copper could change the colour shade of dyestuff of jackfruit stems.