To study the parameters affected on the preparation of MEA for PEM fuel cell. The MEA was prepared by using 5 sq.m. of porous electrodes with Pt loading 1 mg.sq.cm and nafion membrane from Electrochem company. The studied parameters were temperature, pressure and time of compression in the range of 130-150 ํC, 50-100 kg/sq.cm. and 1-5 minutes respectively. 2[superscript k] factorial of experimental design was used in this work. To test the efficiency, PEM single cell was fabricated by using the prepared MEA. The results showed that pressure, temperature, interaction between pressure and temperature and interaction between temperature and time of compression have significant effects on the performance of the MEA. When low pressure, but high temperature and long time of compression, current density is increased. To determine the optimum condition for MEA fabrication two methods were used; varying the values of parameters and response surface method (RSM). The results showed that the optimum condition for MEAfabrication was 65 kg/sq.cm., 137 ํC and 5.5 min of compression time. It was also found that the single cell of PEM fuel cell fabricated with good contact between membrane and electrode the force of 60 lb[subscript 1-] in gave the best performance